Beta Tubulin (Schizosaccharomyces pombe)

AS21 4556 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Schizosaccharomyces pombe

Product Information

Immunogen KLH-conjugated C-terminal peptide C-YEIEEEKEPLEY-OH from beta tubulin of Schizosaccharomyces pombe, UniProt: P05219

Host Rabbit

Clonality

Polyclonal

Purity Immunogen affinity purified serum in PBS and 50 % glycerol, filter sterilized.

Format Liquid

Quantity 100 µg

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 51 | 45 kDa

Reactivity

Confirmed reactivity Schizosaccharomyces pombe

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-beta tubulin (yeast) antibodies

10 µg of a crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in a 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Background

Background Tubulin is the major constituent of microtubules. There are three members (alpha, beta and gamma) and two subtypes in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.

Product citations

Selected references Fedyanina et al. (2009) Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009 Apr;26(4):235-47. doi: 10.1002/yea.1663. PMID: 19330768; PMCID: PMC5705012.

Related products: Beta Tubulin (Schizosaccharomyces pombe)

Agrisera ECL kit (Bright/SuperBright)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...

From 26 €

Info

Add to cart

Goat anti-Rabbit IgG (H&L), HRP conjugated

AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...

201 €

Rad22 | DNA repair and recombination protein rad22 (Saccharomyces pombe)

AS21 4554 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces...

331 €

RAD51 | DNA repair protein RAD51 (Saccharomyces cerevisiae) (ChIP grade)

AS21 4549 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Sacchar...

331 €

Confirmed reactivity:	Schizosaccharomyces pombe
predicted reactivity:	Species of your interest not listed? Contact us
calculated \| apparent molecular mass [kDa]:	51 \| 45 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Rabbit
immunogen:	KLH-conjugated C-terminal peptide C-YEIEEEKEPLEY-OH from beta tubulin of Schizosaccharomyces pombe, UniProt: P05219
Purity:	Immunogen affinity purified serum in PBS and 50 % glycerol, filter sterilized.
Quantity:	100 µg
recommended dilution:	1 : 1000 (WB)
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Picture (footer):	10 µg of a crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in a 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
background:	Tubulin is the major constituent of microtubules. There are three members (alpha, beta and gamma) and two subtypes in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.
All references:	Fedyanina et al. (2009) Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009 Apr;26(4):235-47. doi: 10.1002/yea.1663. PMID: 19330768; PMCID: PMC5705012.