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Beta Tubulin (Schizosaccharomyces pombe)

AS21 4556 | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Schizosaccharomyces pombe

Beta Tubulin (Schizosaccharomyces pombe) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4556)

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Product Information

Immunogen KLH-conjugated C-terminal peptide C-YEIEEEKEPLEY-OH from beta tubulin of Schizosaccharomyces pombe, UniProt: P05219
Host Rabbit
Clonality

Polyclonal

Purity Antigen affinity purified serum, in PBS and 50 % glycerol, filter sterilized
Format

Liquid

Quantity 100 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW 51 | 45 kDa

Reactivity

Confirmed reactivity Schizosaccharomyces pombe
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-beta tubulin (yeast) antibodies


10 µg of a crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in a 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Related products

Background

Background Tubulin is the major constituent of microtubules. There are three members (alpha, beta and gamma) and two subtypes in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.

Product citations

Selected references Fedyanina et al. (2009) Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009 Apr;26(4):235-47. doi: 10.1002/yea.1663. PMID: 19330768; PMCID: PMC5705012.

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