Anti-Beta Tubulin (Schizosaccharomyces pombe)

Product no: AS21 4556

AS21 4556 | Clonality:  Polyclonal |  Host:  Rabbit | Reactivity: Schizosaccharomyces pombe

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated C-terminal peptide C-YEIEEEKEPLEY-OH from beta tubulin of Schizosaccharomyces pombe, UniProt: P05219
    Host: Rabbit


    Purity: Immunogen affinity purified serum in PBS and 50 % glycerol, filter sterilized.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 51 | 45 kDa
  • Reactivity
  • Confirmed reactivity: Schizosaccharomyces pombe
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Western blot using anti-beta tubulin (yeast) antibodies

    10 µg of a crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a PVDF membrane, followed by blocking in a 5 % non-fat milk for 1h/RT. The primary antibody was incubated at 1: 1000 for 1h/RT, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. The reaction was developed using chemiluminescence following manufacture's recommendations.
  • Background
  • Background: Tubulin is the major constituent of microtubules. There are three members (alpha, beta and gamma) and two subtypes in the tubulin family. Of these members, beta tubulin (449 aa, 51 kDa) is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly during cell cycle.
  • Product Citations
  • Selected references: Fedyanina et al. (2009) Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells. Yeast. 2009 Apr;26(4):235-47. doi: 10.1002/yea.1663. PMID: 19330768; PMCID: PMC5705012.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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