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Rad22 | DNA repair and recombination protein rad22 (Saccharomyces pombe)

AS21 4554 | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Saccharomyces pombe

Rad22 | DNA repair and recombination protein rad22 (Saccharomyces pombe) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS21 4554)

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Product Information

Immunogen Purified, full length, recombinant Rad22 protein from Saccharomyces cerevisiae, UniProt: P36592 overexpressed in E. coli
Host Rabbit
Clonality Polyclonal
Purity Antigen purified serum, in PBS with 50 % glycerol, filter sterilized
Format Liquid
Quantity 50 µg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunoprecipiatation (IP), Western blot (WB)
Recommended dilution 1 : 2000 - 1: 5000 (WB)
Expected | apparent MW 52 | 58 kDa

Reactivity

Confirmed reactivity Saccharomyces pombe
Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-Rad22 (yeast) antibodies

Whole cell extract of Saccharomyces pombe was separated on SDS-PAGE and blotted to a membrane. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Western blot using anti-Rad22 (yeast) antibodies

Whole cell extract of Saccharomyces pombe wild-type (1) and Saccharomyces pombe Rad22 deletion mutant (2) was separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

Additional information

Related products

Background

Background Rad22 protein of Schizosaccharomyces pombe (469 aa, 52 kDa) is a functional and structural homologue of Saccharomyces cerevisiae and human Rad52 proteins, which play a major role together with Rhp51 in genetic recombination and recombination repair, by mediating strand annealing reaction between homologous DNA strands.

Product citations

Selected references Lehmann (1996). Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe. Mutat Res. 1996 Aug 8;363(3):147-61. doi: 10.1016/0921-8777(96)00017-1. PMID: 8765156.

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