Rad22 | DNA repair and recombination protein rad22 (Saccharomyces pombe)
AS21 4554 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces pombe
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Product Information
Immunogen
Purified, full length, recombinant Rad22 protein from Saccharomyces cerevisiae, UniProt: P36592 overexpressed in E. coli
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum, in PBS. Contains 50 % glycerol, filter sterilized.
Format
Liquid
Quantity
50 ĩg
Storage
Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipiatation (IP), Western blot (WB)
Recommended dilution
1 : 2000 - 1: 5000 (WB)
Expected | apparent MW
52 | 58 kDa
Reactivity
Confirmed reactivity
Saccharomyces pombe
Predicted reactivity
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Application examples
Application examples
Whole cell extract of Saccharomyces pombe was separated on SDS-PAGE and blotted to a membrane. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Whole cell extract of Saccharomyces pombe wild-type (1) and Saccharomyces pombe Rad22 deletion mutant (2) was separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Whole cell extract of Saccharomyces pombe was separated on SDS-PAGE and blotted to a membrane. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Whole cell extract of Saccharomyces pombe wild-type (1) and Saccharomyces pombe Rad22 deletion mutant (2) was separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Additional information
Background
Background
Rad22 protein of Schizosaccharomyces pombe (469 aa, 52 kDa) is a functional and structural homologue of Saccharomyces cerevisiae and human Rad52 proteins, which play a major role together with Rhp51 in genetic recombination and recombination repair, by mediating strand annealing reaction between homologous DNA strands.
Product citations
Selected references
Lehmann (1996). Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe. Mutat Res. 1996 Aug 8;363(3):147-61. doi: 10.1016/0921-8777(96)00017-1. PMID: 8765156.
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