Rad22 | DNA repair and recombination protein rad22 (Saccharomyces pombe)

Product no: AS21 4554

AS21 4554 | Clonality: Polyclonal |  Host: Rabbit  | Reactivity: Saccharomyces pombe

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  • Product Info
  • Immunogen: Purified, full length, recombinant Rad22 protein from Saccharomyces cerevisiae, UniProt: P36592 overexpressed in E. coli
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum, in PBS. Contains 50 % glycerol, filter sterilized.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunofluorescence (IF), Immunoprecipiatation (IP), Western blot (WB)
    Recommended dilution: 1 : 2000 - 1: 5000 (WB)
    Expected | apparent MW: 52 | 58 kDa
  • Reactivity
  • Confirmed reactivity: Saccharomyces pombe
    Predicted reactivity: Species of your interest not listed? Contact us
  • Application Examples
  • Western blot using anti-Rad22 (yeast) antibodies

    Whole cell extract of Saccharomyces pombe was separated on SDS-PAGE and blotted to a membrane. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.

    Western blot using anti-Rad22 (yeast) antibodies

    Whole cell extract of Saccharomyces pombe wild-type (1) and Saccharomyces pombe Rad22 deletion mutant (2) was separated on a 12.5 % SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
  • Background
  • Background: Rad22 protein of Schizosaccharomyces pombe (469 aa, 52 kDa) is a functional and structural homologue of Saccharomyces cerevisiae and human Rad52 proteins, which play a major role together with Rhp51 in genetic recombination and recombination repair, by mediating strand annealing reaction between homologous DNA strands.
  • Product Citations
  • Selected references: Lehmann (1996). Molecular biology of DNA repair in the fission yeast Schizosaccharomyces pombe. Mutat Res. 1996 Aug 8;363(3):147-61. doi: 10.1016/0921-8777(96)00017-1. PMID: 8765156.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials
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