Rhp51 | DNA repair protein rhp51 (Saccharomyces pombe)
AS21 4553 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Saccharomyces pombe
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Whole cell extract of Saccharomyces pombe (1) and Rhp51 deletion mutant (2) was separated on SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 2000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations.
Saccharomyces pombe cells without (-MMS) and with (+MMS at 0.025 % treatment for 1 h) were subjected to immunofluorescent staining. Primary antibody was used at 1: 500 dilution.
Lambert et al. (2005). Gross chromosomal rearrangements and elevated recombination at an inducible site-specific replication fork barrier. Cell. 2005 Jun 3;121(5):689-702. doi: 10.1016/j.cell.2005.03.022. PMID: 15935756. (Immunoflourescence)
Akamatsu et al. (2003) Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast. Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15770-5. doi: 10.1073/pnas.2632890100. Epub 2003 Dec 8. PMID: 14663140; PMCID: PMC307643. (Immunoprecipitation, Western Blot)
Kibe et al. (2003). Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer. Nucleic Acids Res. 2003 Sep 1;31(17):5054-63. doi: 10.1093/nar/gkg718. PMID: 12930956; PMCID: PMC212814. (ChIP)
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