BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)

Product no: AS20 4465

AS20 4465 | Clonality: Polyclonal  Host: Chicken Reactivity: Botulinum Neurotoxin A

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  • Product Info
  • Immunogen:

    Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)

    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide.
    Format: Liquid
    Quantity: 0,1 mg
    Storage: Store at -20C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1:10 000 (ELISA), 1:2000 (WB)
    Expected | apparent MW: 97 kDa
  • Reactivity
  • Confirmed reactivity: Botulinum toxin A heavy chain
  • Additional Information
  • Additional information (application): For direct ELISA coating with 2 µg of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000.

    For Western blot 0.5 µg of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.
  • Background
  • Background: Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

    Alternative name: Bontoxilysin-A
  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody Protocols
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