BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)

Name: BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)
Brand: IKP
SKU: AS20 4465
Price: 451 EUR
Availability: InStock

AS20 4465 | Clonality: Polyclonal | Host: Chicken | Reactivity: Botulinum Neurotoxin A

BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain) in the group Antibodies Plant/Algal / Toxins / Other compounds at Agrisera AB (Antibodies for research) (AS20 4465)

Product Information

Immunogen

Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide.

Format Liquid

Quantity 0,1 mg

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Western blot (WB)

Recommended dilution 1:10 000 (ELISA), 1:2000 (WB)

Expected | apparent MW 97 kDa

Reactivity

Confirmed reactivity Botulinum toxin A heavy chain

Application examples

Additional information

For direct ELISA coating with 2 µg of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000.

For Western blot 0.5 µg of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.

Background

Background Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

Alternative name: Bontoxilysin-A

Product citations

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immunogen:	Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)
Host:	Chicken
Clonality:	Polyclonal
Purity:	Immunogen affinity purified in PBS pH 7.2. Contains 0.075% sodium azide.
Format:	Liquid
Quantity:	0,1 mg
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Western blot (WB)
recommended dilution:	1:10 000 (ELISA), 1:2000 (WB)
calculated \| apparent molecular mass [kDa]:	97 kDa
Confirmed reactivity:	Botulinum toxin A heavy chain
additional information (application):	For direct ELISA coating with 2 µg of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000. For Western blot 0.5 µg of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.
background:	Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light. Alternative name: Bontoxilysin-A