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BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain)

AS20 4465 | Clonality: Polyclonal  Host: Chicken Reactivity: Botulinum Neurotoxin A

BoNT-A | Botulinum Neurotoxin A (Heavy Chain Binding Domain) in the group Antibodies for Plant/Algal  / Toxins / Other compounds at Agrisera AB (Antibodies for research) (AS20 4465)

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Product Information

Immunogen

Purified Botulinum Neurotoxin Type A (Heavy Chain Binding Domain)

Host Chicken
Clonality Polyclonal
Purity Affinity purified 
Format Liquid in PBS pH 7.2, with 0.075% sodium azide
Quantity 0.1 mg
Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1:10 000 (ELISA), 1:2000 (WB)

Reactivity

Confirmed reactivity  Botulinum toxin A heavy chain

Application examples

Additional information

For direct ELISA coating with 2 µg of Botulinium Toxin A is recommended combined with primary antibody dilution of 1: 10 000.

For Western blot 0.5 µg of non reduced holotoxin or heavy chains is recommended together with 1: 2000 dilution of a primary antibody.

Related products

Related products

AS16 4087 | Anti-BoNT-B | Botulinum toxin B, rabbit antibodies
AS16 4088 | Anti-BoNT-E | Botulinum toxin E, rabbit antibodies

Background

Background Botulinum toxin is a toxin produced by the anaerobic, gram-positive, bacterium of the genus Clostridium (C. botulinum, C. butyricum, C. baratii and C. argentinense). These strains are widely distributed and can be found in soil and dust. Eight types of botulinum toxin are distinguished, named type A–H. Type A and B are capable of causing disease in humans (botulism) and have longest activity in vivo, and are also used commercially (BOTOX) and medically. Types C–G are less common; types E and F can cause disease in humans, while the other types cause disease in other animals. BotA is cleaved into two chains: heavy and light.

Alternative name: Bontoxilysin-A

Product citations

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