Bromelain, Biotin conjugated

AS09 553 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus

Product Information

Immunogen

native bromelain isolated and purified from Ananas comosus

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ananas comosus

Predicted reactivity Ananas comosus

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.