Bromelain, Biotin conjugated

Name: Bromelain, Biotin conjugated
Brand: IKP
SKU: AS09 553
Price: 401 EUR
Availability: InStock

AS09 553 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus

Bromelain, Biotin conjugated in the group Antibodies Plant/Algal / Food Proteins/Allergens at Agrisera AB (Antibodies for research) (AS09 553)

Product Information

Immunogen

native bromelain isolated and purified from Ananas comosus

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Ananas comosus

Predicted reactivity Ananas comosus

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Background

Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.

Product citations

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immunogen:	native bromelain isolated and purified from Ananas comosus
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Purified IgG in PBS.
Format:	Lyophilized
Quantity:	10 mg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
recommended dilution:	1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)
Confirmed reactivity:	Ananas comosus
predicted reactivity:	Ananas comosus
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
background:	Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.