Bromelain, Biotin conjugated
AS09 553 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Ananas comosus
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native bromelain isolated and purified from Ananas comosus
Purity Purified IgG in PBS.
Quantity 10 mg
Reconstitution For reconstitution add 1 ml of sterile destilled water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 5000-1 : 50 000 (ELISA), (IF), (IHC), (WB)
Confirmed reactivity Ananas comosus
Predicted reactivity Ananas comosus
Not reactive in No confirmed exceptions from predicted reactivity are currently known
Additional information Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added
Bromelain (EC=18.104.22.168) is a cysteine protease isolated from pineapple stem.
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