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CCA1 | Circadian clock associated 1

AS13 2659 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

CCA1 | Circadian clock associated 1 in the group Antibodies for Plant/Algal  / Developmental Biology / Circadian clock at Agrisera AB (Antibodies for research) (AS13 2659)

DATA SHEET IN PDF

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CCA1 protein sequence, UniProt:P92973, TAIR:AT2G46830

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Co-immunoprecipitation (Co-IP), Western blot (WB)
Recommended dilution 1: 500 (Co-IP), 1 : 500 (WB)
Expected | apparent MW

67 | 80 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

western blot using anti-CCA1 antibodies

A clear band for CCA1 was detected at about 80kDa after electrophoresis using a 8% polyacrylamid gel cast in a Biorad gel device with 15 wells and 1mm thickness. Nuclear extract of Arabidopsis thaliana Ws (Wasilewska), zt0 zt6 and lhy/cca1/toc1 zt0 was analyzed and protein concentration was equalized according to Bradford quantification to ~10µg proteins per lane after resuspending and heating to 100°C for 10 min in SDS loading buffer. Proteins were well separated on the PAGE, the gels were equilibrated for 10 min in blotting buffer of 29g/L Glycine, 5,9g/L Tris base with 20% MEOH. Proteins were transferred in a wet western transfer device from Biorad for 12 h on a PVDF membrane. >From 50µg/50µl stock solution the antibody was diluted 1:500 in 1X TBS and incubated with or without 10µg/ml of peptide for 1h at RT followed by two hours incubation on separated parts of the same blot. Washing the unspecific bound antibody from the blot with TBS 0.1% tween for one hour changing solutions twice after the incubation. Secondary antibody was goat anti-rabbit HRP conjugated (AS09 602) used at 1:5000 in TBS with 5% milk for two hours shaking at RT. With TBS Tween unspecific bond antibody was washed of for one hour changing solution twice. For the last Wash TBS without tween was used for about 10 minutes. As a substrate for the HRP,  the most sensitive chemilumininescent detection reagent was used in extreme low femtogram range. Pictures were taken with 10sec exposure and increment setting. After 3 pictures taken a clear band was detectable.

Courtesy of Dr. Mark Ruhl, Umeå Plant Science Centre, Sweden

Additional information

Important note about protein extraction
Transcription factors are best isolated by freezing nucelar extract in liquid nitrogen after addition of extraction buffer, followed by thawing directly at 100°C heating block, not at RT. Centrifugation for 5 minutes will remove cell debris. Protein concenration is measure ad rest is frozen in liquid Nitrogen, in aliquots. Frozen samples are thawed at RT and loaded as soon as they are in liquid form, without heating. Such precautions are necessary to take to be able to detect a transcription factor using antibodies.

Related products

Related products

AS13 2661 | Anti-LHY | Late elongated hypocotyl, rabbit antibodies
AS13 2646 | Anti-TOC1 | TIMING OF CAB EXPRESSION 1, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background CCA1 (Circadian clocl associated 1) encodes a transcriptional repressor that performs overlapping functions with LHY in a regulatory feedback loop that is closely associated with the circadian oscillator of Arabidopsis. Binds to the evening element in the promoter of TOC1 and represses TOC1 transcription. CCA1 and LHY colocalize in the nucleus and form heterodimers in vivo. CCA1 and LHY function synergistically in regulating circadian rhythms of Arabidopsis.

Product citations

Selected references Hung er al. (2018). The Arabidopsis LDL1/2-HDA6 histone modification complex is functionally associated with CCA1/LHY in regulation of circadian clock genes. Nucleic Acids Res. 2018 Aug 14. doi: 10.1093/nar/gky749.

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