CNX1/2 | CALNEXIN HOMOLOG 1/2

AS12 2365 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana CNX1 UniProt: P29402 TAIR: AT5G61790, CNX2 UniProt: Q38798, TAIR: AT5G07340. This peptide is NOT present in calreticulins.

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Liquid

Quantity 50 µg

Storage

Aliquite upon arrival to avoid repeated freeze-thaw cycles and store -20°C; Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2500 (WB)

Expected | apparent MW

CNX1 60.5 kD, processing aa 1-20, mature peptide 58.1 kD

CNX2 60.5/61.4 kD, processing aa 1-25, mature peptides 57.6/58.6 kD

Reactivity

Confirmed reactivity Arabidopsis thaliana, Nicotiana tabacum, Petunia hybrida, Solanum lycopersicum

Predicted reactivity Brassica napus, Coccomyxa suellipsoidea, Hordeum vulgare, Glycine max, Medicago truncatula, Oryza sativa, Petunia inflata, Physcomitrella patens, Picea sitcHensis, Pisum sativum, Populus trichocarpa, Ricinus communis, Stereum hirsutum, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

Total protein from Col-0 (wild-type) Arabidopsis thaliana were extracted with 50mM HEPES-KOH buffer containing 250 mM sucrose, 5% glycerol, 50 mM NaPP, 1 mM NaMo, 25 mM NaF, 10mM EDTA, 0.5% PVP, 3mM DTT, 1mM PMSF, 10uM Leupeptin & 10nM Calyculin, and then fractionated by ultracentrifugation at 100,000 x gravity for 30 min at 4°C into soluble (S100) and microsomal (P100) proteins as described in LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Current Protocols in Plant Biology 1:1-18. doi: 10.1002/cppb.20020. 30 µg proteins of total, S100 and P100 fractions were denatured at 37°C for 5 min, separated on a 7.5 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 1x PBS (from Fisher Scientific BP665-1) + 0.1 %Tween 20 (PBS-T) + 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2500 overnight at 4°C with agitation in 1x PBS-T + 5% milk. The antibody solution was decanted, and the blot was rinsed briefly once, then washed four times for 7 min in 1x PBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in 1x PBS-T + 5% milk for 2 hrs at RT with agitation. The blot was washed as above and developed for 4 min with Amersham ECL (RPN2106). Exposure time was 30 seconds and 2 min

Courtesy of Erica LaMontagne & Dr. Antje Heese (Division of Biochemistry, Interdisciplinary Plant Group (IPG) - University of Missouri; Columbia, MO, USA)

Additional information

Antibody can be used as a marker of microsmal membrane.

Related products

Related products AS17 4164 | Anti-CNX | Calnexin (monoclonal) antibody

Plant protein extraction buffer

Secondary antibodies

Background

CNX1/2 (calnexin homolog 1/2) is a calcium-binding protein involved in protein folding. It interacts with newly synthesized glycoproteins in the endoplasmic reticulum.

Product citations

Selected references Kramer et al. (2020). N6-methyladenosine and RNA secondary structure affect transcript stability and protein abundance during systemic salt stress in Arabidopsis. Plant Direct . 2020 Jul 24;4(7):e00239.doi: 10.1002/pld3.239.
Collins et al. (2020). EPSIN1 Modulates the Plasma Membrane Abundance of FLAGELLIN SENSING2 for Effective Immune Responses . Plant Physiol. 2020 Feb 24. pii: pp.01172.2019. doi: 10.1104/pp.19.01172
Butler et al. (2019). Soybean resistance locus Rhg1 confers resistance to multiple cyst nematodes in diverse plant species. Phytopathology. 2019 Aug 12. doi: 10.1094/PHYTO-07-19-0225-R.
Howden et al. (2017), Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity. New Phytol. 2017 Jul;215(1):309-322. doi: 10.1111/nph.14540.
Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
LaMontagne et al. (2016). Isolation of Microsomal Membrane Proteins from Arabidopsis thaliana. Curr. Protoc. Plant Biol. 1:217-234. doi: 10.1002/cppb.20020.