COR14b | Cor14b-encoded cold regulated protein

KLH-conjugated synthetic peptide conserved in cor14b sequence of Hordeum vulgare, cor14c UniProt Q8H0Q4 and Triticum aestivum, REP13 of Secale cereale 

16 kDa

Application example

Total cell extract of (1-3) Hordeum vulgare  after 1 day of cold acclimation (4-6) Hordeum vulgare  after 6 hours of cold acclimation, (7-9) Hordeum vulgare  non-acclimated plants were separated on  SDS-PAGE. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation.  Blots were developed using chemiluminescent detection reagent. 

collection of antibodies to various plant stress proteins

Plant protein extraction buffer

Secondary antibodies

Cor14b is a nuclear encoded protein localized in chloroplast whose expression level has been associated with frost and photoinhibition tolerance in monocots.