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Anti-COR14b | Cor14b-encoded cold regulated protein
AS06 169 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Monocots including Hordeum vulgare
- Product Info
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Immunogen: KLH-conjugated synthetic peptide conserved in cor14b sequence of Hordeum vulgare, cor14c UniProt Q8H0Q4 and Triticum aestivum, REP13 of Secale cereale
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 100 µl Reconstitution: For reconstitution add 100 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 10 000 (WB) Expected | apparent MW: 16 kDa
- Reactivity
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Confirmed reactivity: Hordeum vulgare Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
Total cell extract of (1-3) Hordeum vulgare after 1 day of cold acclimation (4-6) Hordeum vulgare after 6 hours of cold acclimation, (7-9) Hordeum vulgare non-acclimated plants were separated on SDS-PAGE. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. Blots were developed using chemiluminescent detection reagent.
- Background
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Background: Cor14b is a nuclear encoded protein localized in chloroplast whose expression level has been associated with frost and photoinhibition tolerance in monocots.
- Product Citations
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Selected references: Rapacz et al. (2008). The effect of cold acclimation on photosynthetic apparatus and the expression of COR14b in four genotypes of barley (Hordeum vulgare) contrasting in their tolerance to freezing and high light treatment in cold. Ann. Bot. 101: 689-699.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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