CPX1 | coproporphyrinogen III oxidase, isoform 1
AS06 123 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii, Physcomitrella patens
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Product Information
Immunogen
residues 32-366 from mature coproporphyrinogen III oxidase, isoform CPX1 of Chlamydomonas reinhardtii fused to TrxA Q9S7V1
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
100 µl
Reconstitution
For reconstitution add 100 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 3000 (WB)
Expected | apparent MW
41.4 | 38 kDa
Reactivity
Confirmed reactivity
Physcomitrella patens
Predicted reactivity
Arabidopsis thaliana, Zea mays
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Additional information
Background
Background
Coproporphyrinogen III oxidase is an enzyme in the biosynthesis of tetrapyrroles. This isoform is encoded by a single nuclear gene in Chlamydomonas reinhardtii. The abundance of the protein increases in copper deficient cells. The protein is localized to the chloroplast.
Product citations
Selected references
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Quinn et al. (1999) Induction of Coproporphyrinogen Oxidase in Chlamydomonas Chloroplasts Occurs via Transcriptional Regulation of Cpx1 Mediated by Copper-Response Elements and Increased Translation from a Copper-Deficiency-Specific Form of the Transcript. J. Biol. Chem. 274:14444-14454.
Quinn et al. (1999) Induction of Coproporphyrinogen Oxidase in Chlamydomonas Chloroplasts Occurs via Transcriptional Regulation of Cpx1 Mediated by Copper-Response Elements and Increased Translation from a Copper-Deficiency-Specific Form of the Transcript. J. Biol. Chem. 274:14444-14454.
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