CPX1 | coproporphyrinogen III oxidase, isoform 1

Product no: AS06 123

AS06 123  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii, Physcomitrella patens

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  • Product Info
  • Immunogen:

    residues 32-366 from mature coproporphyrinogen III oxidase, isoform CPX1 of Chlamydomonas reinhardtii fused to TrxA Q9S7V1

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 100 l
    Reconstitution: For reconstitution add 100 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 3000 (WB)
    Expected | apparent MW: 41,4 | 38 kDa
  • Reactivity
  • Confirmed reactivity: Physcomitrium patens
    Predicted reactivity: Arabidopsis thaliana, Zea mays
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Background
  • Background:

    Coproporphyrinogen III oxidase is an enzyme in the biosynthesis of tetrapyrroles. This isoform is encoded by a single nuclear gene in Chlamydomonas reinhardtii. The abundance of the protein increases in copper deficient cells. The protein is localized to the chloroplast.

  • Product Citations
  • Selected references: Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
    Quinn et al. (1999) Induction of Coproporphyrinogen Oxidase in Chlamydomonas Chloroplasts Occurs via Transcriptional Regulation of Cpx1 Mediated by Copper-Response Elements and Increased Translation from a Copper-Deficiency-Specific Form of the Transcript. J. Biol. Chem. 274:14444-14454.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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