CpxA | Conjugative plasmid expression
AS13 2653 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Yersinia pseudotuberculosis

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Product Information
Recombinant CpxA (residues 26 to 168) over-expressed as a N-terminal fusion with His(x6)-tag, which was removed prior to immunization. The antigen originates from enteropathogenic Yersinia pseudotuberculosis YPIII; B1JQV1, NCBI annotated locus tag YPK_4133.
Reactivity
Application examples
application example
Lanes: Marker: PageRuler™Plus Prestained Ladder (ThermoScientific)
Wildtype: Y. pseudotuberculosis YPIII/pIB102
cpxA: Y. pseudotuberculosis YPIII07/pIB102 (cpxA in frame deletion of codons 41 to 449) [Carlsson et al., Infect. Immun., 75 (2007), pp. 3913–3924]
cpxA101*: Y. pseudotuberculosis YPIII/pIB102 (cpxA allele encoding for the substitution of T253P) [Liu et al., PLoS One, 6 (2011), p. e23314]
Courtesy of Dr. Matthew Francis, Umeå University, Sweden
Additional information
Background
CpxA (Conjugative Plasmid Expression) is the 458 amino acid protein, a bacterial inner membrane histidine protein kinase, responsible for sensing misfolded proteins in the periplasm. It is part of CpxA/CpxR two component signal transduction system, where CpxR is the cognate response regulator. In sensing bacterial envelope stress, the Cpx pathway regulates the production of a number of periplasmic folding and trafficking factors to maintain outer membrane integrity. It is also responsible for global control of virulence factor expression. Alternative name: Integral membrane sensor signal transduction histidine kinase.
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