DGAT2A | Acyl-CoA: Diacylglycerol acyltransferase

Name: DGAT2A | Acyl-CoA: Diacylglycerol acyltransferase
SKU: AS12 1874
Price: 394 EUR
Availability: InStock

AS12 1874 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

DGAT2A | Acyl-CoA: Diacylglycerol acyltransferase in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS12 1874)

DGAT2A | Acyl-CoA: Diacylglycerol acyltransferase

Product Information

Immunogen recombinant CrDGAT2A, overexpressed in E.coli, missing transmembrane domains, PID 536226, also annotated as DGTT1 UniProt: A8JGY1

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

application example

Total proteins (containing 30 ug ) from Chlamydomonas reinhardtii cells grown for the indicated times in N-deprived medium extracted with lysis buffer (50 mM Tris-HCl, pH 6.8, containing 2% SDS and 10 mM EDTA and a protease inhibitor cocktail) were separated on 12 % SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powder in TBS-T) for 2 hrs at room temperature (RT) with agitation. Blots were incubated in the primary antibody (CrTMDGAT2A) was used at a dilution of 1:1000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then whashed 5 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5000 in the same buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Chemiluminescence detection kit according to the manufacturers instructions. An imaging system (ChemiDoc XRS; Bio-Rad) was used to quantitatively and qualitatively analyze protein blot. Exposure time was 30 seconds.

Courtesy Dr. Yantao Li, The University of Maryland, USA

Additional information

Background

Background DGAT2A (Acyl-CoA: Diacylglycerol acyltransferase) is an enzyme which exhibits transferase activity, transferring acyl groups. EC=2.7.8.2.

Product citations

Selected references Liu et al. (2016). A simple and reproducible non-radiolabeled in vitro assay for recombinant acyltransferases involved in triacylglycerol biosynthesis. J Appl Phycol (2016). doi:10.1007/s10811-016-0949-6.
Wase et al. (2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii. Algal Research, Volume 11, September 2015, Pages 74–84.

immunogen:	recombinant CrDGAT2A, overexpressed in E.coli, missing transmembrane domains, PID 536226, also annotated as DGTT1 UniProt: A8JGY1
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	100 µl
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Western blot (WB)
recommended dilution:	1 : 1000 (WB)
Expected \| apparent MW:	kDa
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Chlamydomonas reinhardtii
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	application example Total proteins (containing 30 ug ) from Chlamydomonas reinhardtii cells grown for the indicated times in N-deprived medium extracted with lysis buffer (50 mM Tris-HCl, pH 6.8, containing 2% SDS and 10 mM EDTA and a protease inhibitor cocktail) were separated on 12 % SDS-PAGE and transferred onto a nitrocellose blot over night at 4°C. Blots were blocked with blocking buffer ( 5% (w/v) non-fat dry milk powder in TBS-T) for 2 hrs at room temperature (RT) with agitation. Blots were incubated in the primary antibody (CrTMDGAT2A) was used at a dilution of 1:1000 over night at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then whashed 5 times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:5000 in the same buffer for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Chemiluminescence detection kit according to the manufacturers instructions. An imaging system (ChemiDoc XRS; Bio-Rad) was used to quantitatively and qualitatively analyze protein blot. Exposure time was 30 seconds. Courtesy Dr. Yantao Li, The University of Maryland, USA
background:	DGAT2A (Acyl-CoA: Diacylglycerol acyltransferase) is an enzyme which exhibits transferase activity, transferring acyl groups. EC=2.7.8.2.
All references:	Liu et al. (2016). A simple and reproducible non-radiolabeled in vitro assay for recombinant acyltransferases involved in triacylglycerol biosynthesis. J Appl Phycol (2016). doi:10.1007/s10811-016-0949-6. Wase et al. (2015). Phenotypic screening identifies Brefeldin A/Ascotoxin as an inducer of lipid storage in the algae Chlamydomonas reinhardtii. Algal Research, Volume 11, September 2015, Pages 74–84.