CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C

AS14 2760  |  Clonality:Polyclonal  |  Host:Rabbit  |  Reactivity: proteobacteria

CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C in the group Plant/Algal Antibodies / Photosynthesis  / RUBISCO/Carbon metabolism at Agrisera AB (Antibodies for research) (AS14 2760)


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product information
Background Major carboxysome shell protein is involved in the formation of the carboxysome, polyhedral includion sequestering Rubisco.

KLH-conjugated peptide conserved in Major carboxysome shell protein 1A, UniProt: P45689,  Major carboxysome shell protein 1B
P45690, Major carboxysome shell protein 1C, UniProt:

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products Collection of antibodies to cyanobacteria
Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

9-11 kDa

Confirmed reactivity Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus)
Predicted reactivity Algae (red), Cyanobacteria, Cryptomonads
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references To be added when available, antibody released in February 2015

application example

western blot using anti CsoS1 antibodies

0.125 - 1.0 µg of total purified Halothiobacillus neapolitanus carboxysome protein (equivalent to approximately 20 - 170 ng CsoS1A, B and C proteins) were separated on 4-20 % Bio-Rad TGX stain-free SDS-PAGE and blotted 1h to PVDF using a Bio-Rad TransBlot Turbo Blotting System. Blots were blocked with 5% skim milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat-anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:5 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and antibody binding determined using the Promega Attophos reagent system as described by the manufacturer. Reaction time was 10 seconds and membranes were imaged using a Bio-Rad VersaDoc.

Courtesy of Dr. Ben Long, The Australian National University, Australia

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