CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C
AS14 2760 | Clonality:Polyclonal | Host:Rabbit | Reactivity: proteobacteria
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0.125 - 1.0 µg of total purified Halothiobacillus neapolitanus carboxysome protein (equivalent to approximately 20 - 170 ng CsoS1A, B and C proteins) were separated on 4-20 % Bio-Rad TGX stain-free SDS-PAGE and blotted 1h to PVDF using a Bio-Rad TransBlot Turbo Blotting System. Blots were blocked with 5% skim milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat-anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:5 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and antibody binding determined using the Promega Attophos reagent system as described by the manufacturer. Reaction time was 10 seconds and membranes were imaged using a Bio-Rad VersaDoc.
Courtesy of Dr. Ben Long, The Australian National University, Australia
Li et al. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020 Oct 28;11(1):5448. doi: 10.1038/s41467-020-19280-0. PMID: 33116131; PMCID: PMC7595155.
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
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