CsoS1A/B/C | Major carboxysome shell protein 1A, AB, 1C
AS14 2760 | Clonality:Polyclonal | Host:Rabbit | Reactivity: proteobacteria

Data sheet | Product citations | Customer reviews |
Product Information
Immunogen
KLH-conjugated peptide conserved in Major carboxysome shell protein 1A, UniProt: P45689, Major carboxysome shell protein 1B UniProt: P45690, Major carboxysome shell protein 1C, UniProt:
P45688
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 µg
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 5000 (WB)
Expected | apparent MW
9-11 kDa
Reactivity
Confirmed reactivity
Halothiobacillus neapolitanus (strain ATCC 23641 / c2) (Thiobacillus neapolitanus)
Predicted reactivity
Algae (red), Cyanobacteria, Cryptomonads
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Synechococcus elongatus
Application examples
Application examples
application example

0.125 - 1.0 µg of total purified Halothiobacillus neapolitanus carboxysome protein (equivalent to approximately 20 - 170 ng CsoS1A, B and C proteins) were separated on 4-20 % Bio-Rad TGX stain-free SDS-PAGE and blotted 1h to PVDF using a Bio-Rad TransBlot Turbo Blotting System. Blots were blocked with 5% skim milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat-anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:5 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and antibody binding determined using the Promega Attophos reagent system as described by the manufacturer. Reaction time was 10 seconds and membranes were imaged using a Bio-Rad VersaDoc.
Courtesy of Dr. Ben Long, The Australian National University, Australia

0.125 - 1.0 µg of total purified Halothiobacillus neapolitanus carboxysome protein (equivalent to approximately 20 - 170 ng CsoS1A, B and C proteins) were separated on 4-20 % Bio-Rad TGX stain-free SDS-PAGE and blotted 1h to PVDF using a Bio-Rad TransBlot Turbo Blotting System. Blots were blocked with 5% skim milk powder in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat-anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:5 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and antibody binding determined using the Promega Attophos reagent system as described by the manufacturer. Reaction time was 10 seconds and membranes were imaged using a Bio-Rad VersaDoc.
Courtesy of Dr. Ben Long, The Australian National University, Australia
Additional information
Background
Background
Major carboxysome shell protein is involved in the formation of the carboxysome, polyhedral includion sequestering Rubisco.
Product citations
Selected references
Huang, Jiang, Yang, et al. (2022) Probing the Internal pH and Permeability of a Carboxysome Shell. Biomacromolecules. 2022;23(10):4339-4348. doi:10.1021/acs.biomac.2c00781
Chen et al. (2022) ACS Synth. Biol. 2022, 11, 1, 154-161Publication Date:October 19, 2021 https://doi.org/10.1021/acssynbio.1c00311
Li et al. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020 Oct 28;11(1):5448. doi: 10.1038/s41467-020-19280-0. PMID: 33116131; PMCID: PMC7595155.
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
Chen et al. (2022) ACS Synth. Biol. 2022, 11, 1, 154-161Publication Date:October 19, 2021 https://doi.org/10.1021/acssynbio.1c00311
Li et al. (2020). Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020 Oct 28;11(1):5448. doi: 10.1038/s41467-020-19280-0. PMID: 33116131; PMCID: PMC7595155.
Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
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