CSP41b | ribosome associated endonuclease (CRB)

AS08 298 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum, Physcomitrella patens

CSP41b | ribosome associated endonuclease (CRB) in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS08 298)
CSP41b | ribosome associated endonuclease (CRB)


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Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


KLH-conjugated synthetic peptide derived from CSP41b protein sequence of Arabidopsis thaliana Q9SA52, At1g09340

Host Rabbit
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

42 | 39 kDa


Confirmed reactivity Arabidopsis thaliana, Pisum sativum, Physcomitrium patens
Predicted reactivity Oryza sativa, Picea sitcHenis, Populus sp., Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application information

10 µg of total leaf protein from P.sativum (1) was separated on 4-12% NuPage (Invitrogen) and blotted on nitrocellulose. Molecular marker was MagicMark (Invitrogen). Filters where blocked overnight in TBST containing 5 % milkpowder and incubated with 1:1000 anti-CSP41b antibody (AS08 298, 1h). After incubation with 1:10000 secondary HRP coupled anti-rabbit antibody (1h) signals were visualized with standard chemiluminescen detection reagent.

Additional information

Related products



CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.

Product citations

Selected references Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.
Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.

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