CSP41b | ribosome associated endonuclease (CRB)

355 €

AS08 298 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum, Physcomitrella patens


16 st
Item No:
AS08 298

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product information

CSP41b (CRB, RAP38 ortholog, gb5f) is a chloroplast stem-loop-binding, ribosome-associated endonuclease. This protein is involved in 23S rRNA metabolism and highly conserved in photosynthetic organisms including angio- and gymnosperms, green algae, and cyanobacteria.


KLH-conjugated synthetic peptide derived from CSP41b protein sequence of Arabidopsis thaliana Q9SA52, At1g09340

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to various chloroplastic proteins

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

42 | 39 kDa

Confirmed reactivity Arabidopsis thaliana, Pisum sativum, Physcomitrella patens
Predicted reactivity Oryza sativa, Picea sitcHenis, Populus sp., Zea mays, Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).
Beligni & Mayfield (2008). Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 67:389-401.
Hassidim et al. (2007). Mutations in CHLOROPLAST RNA BINDING provide evidence for the involvement of the chloroplast in the regulation of the circadian clock in Arabidopsis. Plant J. 51:551-562.

application information

10 µg of total leaf protein from P.sativum (1) was separated on 4-12% NuPage (Invitrogen) and blotted on nitrocellulose. Molecular marker was MagicMark (Invitrogen). Filters where blocked overnight in TBST containing 5 % milkpowder and incubated with 1:1000 anti-CSP41b antibody (AS08 298, 1h). After incubation with 1:10000 secondary HRP coupled anti-rabbit antibody (1h) signals were visualized with standard ECL (GE Healthcare).

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