Cyt c6 | Thylakoid lumen cytchrome c6 protein (algal)

AS06 202 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

compartment marker of thylakoid lumen

Cyt c6 | Thylakoid lumen cytchrome c6 protein (algal)

Product Information

Immunogen

GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlorella vulgaris, Cladophora glomerata, Pediastrum duplex
Species of your interest not listed? Contact us

Not reactive in Arabidopsis thaliana, cyanobacteria

Application examples

Additional information

Additional information Cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii

This product can be sold containing proclin if requested.

Note: antibody will work better with nitrocellulose than with PVDF membrane, Recommended load per well min, 6 µg, Primary antibody incubation needs to be done over night, For detection image please refer to the reference below

Background

Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

Product citations

Selected references Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.