Cyt c6 | Thylakoid lumen cytchrome c6 protein

AS06 202 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii | cellular [compartment marker] of thylakoid lumen

Product Information

Immunogen

GST fusion of recombinant Chlamydomonas reinhardtii cytochrome c6 A8J9G6

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity Chlorella vulgaris, Cladophora glomerata, Pediastrum duplex

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information Cellular [compartment marker] of thylakoid lumen for Chlamydomonas reinhardtii

This product can be sold containing proclin if requested.

Note: antibody will work better with nitrocellulose than with PVDF membrane. Recommended load per well min. 6 µg. Primary antibody incubation needs to be done over night. For detection image please refer to the reference below

Related products

AS06 122 | Anti-CRD1 | cyanobacterial homolog of plant CHL27 cyclase, marker of thylakoid and envelope membranes, rabbit antibodies

AS05 084 | Anti-PsbA rabbit antibodies, marker of thylakoid membrane

Collection of antibodies to Chlamydomonas proteins

Background

Cytochrome c6 is known as a redox carrier of the thylakoid lumen of cyanobacteria and some eukaryotic algae that can substitute for plastocyanin in electron transfer.
Cytochrome c6 is situated between the two membrane-bound complexes cytochrome b6f and photosystem I (PSI) in oxygenic photosynthesis.
Cytochrome c6 (cyt c6) in Chlamydomonas reinhardtii is synthesized only under conditions of copper deficiency when plastocyanin cannot be synthesized.

Product citations

Selected references Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
Merchant et al. (1998) Copper-Responsive Gene Expression in Photosynthetic Microorganisms. Methods in Enzymology 297:263-279.