Dehydrin (serum)

AS07 206 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Higher plants

Product Information

Immunogen

KLH-conjugated peptide sequence TGEKKGIMDKIKEKLPGQH of K-segment conserved in a wide range of plant species

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile, deionized water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

9-200 kDa

Reactivity

Confirmed reactivity Agostis stolonifera cv. ‘Penncross’, Betula pubescens, Betula pendula, Betual pendula var. carelica, Hordeum spontaneum, Larix cajanderi, Picea obovata, Picea abies, Pinus sylvestris, Pinus strobus, Pinus sylvestris

Predicted reactivity Arabidopsis thaliana, Glycine max, Nicotiana tabacum, Pisum sativum, Hordeum vulgare, Oryza sativa, Populus sp., Zea mays

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

According to Borovskii et al. 2019, dehydrin detection level can be increased by obtaining a thermostable fraction.

Related products

AS07 206A | Anti-dehydrin (affinity purified), rabbit antibodies

Collection of antibodies to plant stress proteins

AS10 206PRE, Dehydrin, pre-immune serum,to be used as a control for immunolocalization studies

Background

Dehydrins are stress proteins involved in formation of plant protective reactions against dehydration. They are normally synthesized in maturating seeds during their dessication, as well as in vegetative tissues of plants treated with abscisic acid or exposed to environmental stress factors that result in cellular dehydration.

Product citations

Selected references Lv et al. (2018). Characterization of Dehydrin protein, CdDHN4-L and CdDHN4-S, and their differential protective roles against abiotic stress in vitro. BMC Plant Biol. 2018 Nov 26;18(1):299. doi: 10.1186/s12870-018-1511-2.
Maszkowska et al. (2018). Phosphoproteomic analysis reveals that dehydrins ERD10 and ERD14 are phosphorylated by SNF1-related protein kinase 2.10 in response to osmotic stress. Plant Cell Environ. 2018 Oct 19. doi: 10.1111/pce.13465.
Tatarinova et al. (2018). Dehydrins in Buds of Main Birch Species under Conditions of Karelia. Russian Journal of Plant Physiol, Vol 65, Issue 2, pp 295–301.
Tatarinova et al. (2017). Dehydrin stress proteins in birch buds in regions with contrasting climate. ell and Tissue Biology, Volume 11, Issue 6, pp 483–488
Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’)
Jedmowski et al. (2014). Comparative analysis of drought stress effects on photosynthesis of Eurasian and North African genotypes of wild barley. Photosynthetica, September 2014.
Kjellsen et al. (2013). Dehydrin accumulation and extreme low-temperature tolerance in Siberian spruce (Picea obovata). Tree Physiol. 2013 Dec;33(12):1354-66. doi: 10.1093/treephys/tpt105. Epub 2013 Dec 11.
Petrov et al. (2011). Woody plants of Yakutia and low-temperature stress. Russian Journal of Plant Physiology 58:1011-1019.