DET1 | Regulator of the proteasomal degradation of LHY

AS15 3082 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

Recombinant, full length DET1 of Arabidopsis thaliana, overexpressed in E.coli, UniProt: P48732, TAIR: AT4G10180

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 2500 (WB)

Expected | apparent MW

62 | 62 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Capsicum annuum, Glycine max, Medicago truncatula, Oryza sativa, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Theobroma cacao, Zea mays, Zostera marina
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

Recombinant protein (0.5-1-1.5-2 µg of protein) and total proteins from Arabidopsis thaliana whole leaves from plants grown in control light (C), low light (LL) and high light (HL), corresponding to 1 µg of chlorophylls, were extracted with loading buffer (10% glycerol, 62.5 mM Tris pH 6.8, 2% SDS, 5% β-mercaptoethanol) and denatured at 100°C (boiling water) for 1 min. Proteins were separated on 12% SDS-PAGE (Laemly) and blotted 1h to PVDF using tank transfer. Blots were blocked with blocking solution (PBS 1X, 0.2% w/v Tween, 5% powder milk) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody diluted in blocking solution, at a dilution of 1: 25,000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in blocking solution at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG alkaline phosphatase conjugated) diluted to 1:30 000 in blocking buffer for 1h at RT with agitation. The blot was washed 2 times for 10 min in blocking solution and once with PBS 1X solution for 10 min, then developed in developing buffer developing buffer NBT/BCIP by manual agitation.

Courtesy of Stefano Cazzaniga, University of Verona, Italy

Additional information

Additional information This product can be sold containing ProClin if requested.

Related products

AS13 2659 | Anti-CCA1 | Circadian clock assiociated 1, rabbit antibodies

AS13 2661 | Anti-LHY | Late elongated hypocotyl, rabbit antibodies

collection of antibodies to proteins involved in signal transduction

Plant protein extraction buffer

Secondary antibodies

Background

Background DET1 (regulator of the proteasomal degradation of LHY) is a component of light signal transduction (repression of photomorphogenesis in darkness) machinery, localized in nuclei.

Alternative name: Protein DEETIOLATED 1.

Product citations

Selected references Castells et al (2011). The conserved factor DE-ETIOLATED 1 cooperates with CUL4-DDB1DDB2 to maintain genome integrity upon UV stress. EMBO J. 2011 Mar 16;30(6):1162-72. doi: 10.1038/emboj.2011.20. Epub 2011 Feb 8.