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DHAR2 | Dehydroascorbate Reductase 2

AS11 1747 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

DHAR2 | Dehydroascorbate Reductase 2 in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS11 1747)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana Q9FRL8, At1g75270

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Lyophilized
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

23.6 | 23.4 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Ricinus communis, Populus trichocarpa
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

western blot using DHAR2 antibodies

1cm2 of a leaf from Arabidopsis thaliana Col-0 (1) and or t-DNA insertion lines dhar1-1 (2), dhar1-2 (3), dhar1-3 (4), dhar2-1 (5), dhar2-2 (6), dhar1-3 EOS-DHAR1 (7), was extracted using 200µl Lyse&Load-Buffer (Grefen et al. 2009). 10 µl were separated on a 15% SDS-PAGE and blotted 1h to PVDF (using Bjerrum Buffer in a semidry blot). Blots were blocked with 5% Milk in 1xTBS-Tween20 (1%) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:5000 (in 5% Milk 1xTBS-Tween20 (1%) + 0.01 % NaN3) ON at 4°C with agitation. The antibody solution was decanted and the blot was washed 3 times for 10 minutes with 1x TBS-Tween20 at RT with agitation. Blot was incubated in secondary antibody BioRad anti-rabbit IgG AP-conjugate (#170-6518) diluted to 1:2000 in 5% Milk 1xTBS-Tween20 (1%) + 0.01 % NaN3 for 1h at RT with agitation. The blot was washed as above, equilibrated in staining buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, see Grefen et al. 2009) and developed for 5-15 min. with staining solution (Nitro blue tetrazolium chloride (NBT) and 5-bromo-4-chloro-3-indoylphosphate-p-toluidin (BCIP) in staining buffer).

Courtesy Dr. Chrisopher Grefen, UK



Additional information

Related products

Related products

AS11 1746 | Anti-DHAR1, rabbit antibodies

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

DHAR2 ( Dehydroascorbate Reductase 2) the protein is induced by jasmonic acid and oxidative chemical stresses and is a key component of the ascorbate recycling system. Involved in redox homeostasis under biotic and abiotic inducers. Localized in cytoplasm. Synonymes: chloride intracellular channel homolog 2, CLIC homolog 2, glutathione-dependent dehydroascorbate reductase 2, DHAR2, CytDHAR, GSH-dependent dehydroascorbate reductase 2.

Product citations

Selected references Grefen et al. (2009). The determination of protein-protein interactions by the mating-based split-ubiquitin system (mbSUS). Methods Mol Biol 479:217-233.

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