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DYKDDDDK (binds to Sigma FLAGŪ) (mouse monoclonal) (clone F-tag-01)

AS21 4619 | Clonality: Monoclonal | Host: Mouse | Reactivity: DYKDDDDK-Tag
DYKDDDDK (binds to Sigma FLAGŪ) (mouse monoclonal) (clone F-tag-01) in the group Tag Antibodies / DYKDDDDK (binds to SigmaFLAGŪ) at Agrisera AB (Antibodies for research) (AS21 4619)

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Product Information

Immunogen KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®)
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Purity Purified by protein A affinity chromatography
Format Liquid at 1 mg/ml
Quantity 0,1 mg
Storage Store at 2-8°C, Do not freeze, Do not use after the expiration date stamped on the label, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes
Tested applications Immunocyto chemistry (ICC), Western blot (WB)
Recommended dilution 1 µg/ml (WB)

Reactivity

Confirmed reactivity DYKDDDDK (Sigma FLAG®) epitope tag

Application examples

Application examples Immunolocalization using anti-DYKDDDDK (binds to Sigma FLAG®) mouse monoclonal antibodies

COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK (binds to Sigma FLAG®.
A - fusion nuclear protein (red) stained with anti-DYKDDDDK (binds to Sigma FLAG® monoclonal antibodies at 1 µg/ml, secondary antibody goat anti-mouse IgG1 Alexa Fluor® 594
B - cell nuclei stained with DAPI (blue)
C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

Additional information

Additional information This antibody recognizes DYKDDDDK (Sigma FLAGŪ) tagged proteins

Related products

Background

Background Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®). The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Product citations

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