DYKDDDDK (binds to Sigma FLAG®) (mouse monoclonal) (clone F-tag-01)

Product no: AS21 4619
AS21 4619 | Clonality: Monoclonal | Host: Mouse | Reactivity: DYKDDDDK-Tag
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  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide: DYKDDDDK (Sigma FLAG®)
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Immunoglobulin purified on Protein A in PBS pH 7.4. Contains 15 mM sodium azide.
    Format: Liquid at 1 mg/ml.
    Quantity: 0.1 mg
    Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunocyto chemistry (ICC), Western blot (WB)
    Recommended dilution: 1 µg/ml (WB)
  • Reactivity
  • Confirmed reactivity: DYKDDDDK (Sigma FLAG®) epitope tag
  • Application Examples
  • Immunolocalization using anti-DYKDDDDK (binds to Sigma FLAG®) mouse monoclonal antibodies

    COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DYKDDDDK (binds to Sigma FLAG®.
    A - fusion nuclear protein (red) stained with anti-DYKDDDDK (binds to Sigma FLAG® monoclonal antibodies at 1 µg/ml, secondary antibody goat anti-mouse IgG1 Alexa Fluor® 594
    B - cell nuclei stained with DAPI (blue)
    C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

  • Additional Information
  • Additional information: This antibody recognizes DYKDDDDK (Sigma FLAG®) tagged proteins
  • Background
  • Background: Mouse anti-DYKDDDDK is a primary antibody which binds to DYKDDDDK (Sigma FLAG®). The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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