EDS1 | Enhanced disease susceptibility 1

AS13 2751 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana EDS1 sequence, UniProt: Q9SU72, TAIR: AT3G48090

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized in PBS pH 7.4

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)

Recommended dilution 1 : 3000 (WB)

Expected | apparent MW

71.6 kDa | 72 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity Arabidopsis thaliana

Not reactive in

Nicotiana benthamiana

Application examples

Application example

western blot using anti-EDS1 antibodies

20 µg of total protein from Arabidopsis thaliana extracted with HEPES buffer were separated on 8% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5% milk-TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 minutes.

Courtesy of Morgan K. Halane, University of Missouri, USA

Additional information

Additional information This product can be sold containing ProClin if requested

Related products

collection of antibodies to pathogen attack
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Plant protein extraction buffer

Secondary antibodies

Background

EDS1 (Enhanced disease susceptibility 1) is a protein with lipase activity involved in aerencyma formation, lipid metabolic process, response to hypoxia and systemic acquired resistance. Alternative names: Putative disease resistance protein EDS1, Putative uncharacterized protein T17F15.40.

Product citations

Selected references Chang et al. (2019). PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019 Feb 11. pii: S1674-2052(19)30055-3. doi: 10.1016/j.molp.2019.01.023.
Chakraborty et al. (2018). Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen. Plant Science, doi.org/10.1016/j.plantsci.2018.07.014