EDS1 | Enhanced disease susceptibility 1

PRODUCT INFORMATION IN PDF

product information Background EDS1 (Enhanced disease susceptibility 1) is a protein with lipase activity involved in aerencyma formation, lipid metabolic process, response to hypoxia and systemic acquired resistance. Alternative names: Putative disease resistance protein EDS1, Putative uncharacterized protein T17F15.40. Immunogen KLH-conjugated synthetic peptide derived from Arabidopsis thaliana EDS1 sequence, UniProt: Q9SU72, TAIR: AT3G48090 Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized in PBS pH 7.4 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications Western blot (WB) Related products collection of antibodies to pathogen attack collection of antibodies to hypoxia Plant protein extraction buffer Secondary antibodies Additional information

application information
Recommended dilution		1 : 3000 (WB)
Expected | apparent MW		71.6 kDa | 72 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Arabidopsis thaliana
Not reactive in		Nicotiana benthamiana
Additional information
Selected references		to be added when available, antibody released in September 2014.

application example

20 µg of total protein from Arabidopsis thaliana extracted with HEPES buffer were separated on 8% SDS-PAGE and blotted 1h to PVDF using semi-dry or tank transfer. Blots were blocked with 5% milk-TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:25 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 15 minutes.

Courtesy of Morgan K. Halane, University of Missouri, USA

---

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com