EDS1 | Enhanced disease susceptibility 1

Product no: AS13 2751

AS13 2751 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana EDS1 sequence, UniProt: Q9SU72, TAIR: AT3G48090

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 g
    Reconstitution: For reconstitution add 50 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000 (WB)
    Expected | apparent MW: 71,6 kDa | 72 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis thaliana
    Not reactive in: Nicotiana benthamiana, Solanum lycopersicum
  • Application Examples
  • Western blot using anti-EDS1 antibodies

    Samples:
    1 -40 ug of Arabidopsis thaliana whole leaf extract, non treated
    2 -40 ug of Arabidopsis thaliana whole leaf extract, treated with PstAvrB 3h
    3- 40 ug of Arabidopsis thaliana whole leaf extract, old-6 weeks
    4-40 ug of Solanum lycopersicum (negative control) 8 discs extract, control leaf
    5-40 ug of Solanum lycopersicum (negative control) 8 discs extract, wounded leaf
    6-40 ug of Arabidopsis thaliana whole leaf extract, mutant eds1
    7-40 ug of Arabidopsis thaliana whole leaf extract, mutant eds1
    Mark: MW markers: 5uL Thermo scientific pageruler plus prestained ladder

    40 ug/well of total protein was extracted freshly from Arabidopsis thaliana and Solanum lycopersicum (see above) with a buffer: Tris-HCl pH 7,5 50mM; 10% glycerol; 1mM EDTA; 200mM NaCl; 1mM DTT; 0,1% Triton X-100; Sigma protease cocktail; sigma phosphatase inhibitor cocktail; 140 mM pepstatine A and 0,1mM PMSF in MiliQ water (samples 1-5) and Tris-HCl 50mM pH 7.8; 0.2% triton x-100; Sigma protease inhibitor cocktail; 1% PMSF and 1.5% pepstatin A in MiliQ water; (samples 6-7) and denatured with SDS buffer (Tris-HCl pH 6,8, 8% SDS, 40% glycerol) and 1ul DTT 0,5 M at 95°C for 5 min. Proteins were separated on 12 % SDS-PAGE and blotted 1h to PVDF (pore size of 0,45 µm), using semi-dry. Blot was blocked with 3 % non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 with agitation in 3% milk-TBS-T 0,1 %T ON/4°C. The antibody solution was decanted and the blot then washed three times for 10 min 3% milk-TBS-0,1%T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:10 000 in 3% milk-TBS-0,1%T for 1h/RT with agitation. The blot was washed three times wit TBS and developed for 4 min (300uL + 300 uL / membrane) with either Agrisera ECLSuperBright. Exposure time was 1800 seconds in total. The image correspond to 1660-second exposure.

    Courtesy of Dr. María C. Romero-Puertas, Department of Biochemistry, Cell and Molecular Biology of Plants, CSIC, Spain

  • Additional Information
  • Additional information: This product can be sold containing ProClin if requested
  • Background
  • Background:

    EDS1 (Enhanced disease susceptibility 1) is a protein with lipase activity involved in aerencyma formation, lipid metabolic process, response to hypoxia and systemic acquired resistance. Alternative names: Putative disease resistance protein EDS1, Putative uncharacterized protein T17F15.40.

  • Product Citations
  • Selected references: Li et al. (2022) Plasma membrane-nucleo-cytoplasmic coordination of a receptor-like cytoplasmic kinase promotes EDS1-dependent plant immunity. Nat Plants. 2022 Jul;8(7):802-816. doi: 10.1038/s41477-022-01195-x. Epub 2022 Jul 18. PMID: 35851623.
    Chang et al. (2019). PBS3 Protects EDS1 from Proteasome-Mediated Degradation in Plant Immunity. Mol Plant. 2019 Feb 11. pii: S1674-2052(19)30055-3. doi: 10.1016/j.molp.2019.01.023.
    Chakraborty et al. (2018). Epigenetic and transcriptional control of chickpea WRKY40 promoter activity under Fusarium stress and its heterologous expression in Arabidopsis leads to enhanced resistance against bacterial pathogen. Plant Science, doi.org/10.1016/j.plantsci.2018.07.014
  • Protocols

  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection

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Accessories

AS10 687 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. bentamiana, S. oleracea, S. lycopersicum, T. aestivum, Z. mays, V. vinifera

338 €
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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

201 €
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AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206 €
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AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

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