EF-G1 | elongation factor G1

AS13 2652 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Synechocystis sp. PCC6803


product information
Background		EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.
Immunogen		Recombinant EF-G1 protein from Synechocystis sp. PCC6803, P28371, Cyanobase: slr1463
Host		Rabbit
Clonality		Polyclonal
Clone
Purity		Serum
Format		Lyophilized
Quantity		50 µl
Reconstitution		For reconstitution add 50 µl of sterile water.
Storage		Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications		Western blot (WB)
Related products		AS11 1633 \| EF1A \| elongation factor 1-alpha / EF-1-alpha AS10 678 \| eEF1B-alpha1 an 2 \| elongation factor 1B-alpha 1 and 2 AS10 677 \| eEF1B-beta1 and 2 \| elongation factor 1-beta1 and 1-beta2 AS07 265 \| eEF1b \| elongation factor eEF1b-beta protein AS10 676 \| eEF1B-gamma1 and 2 \| elongation factor 1-gamma 1 and collection of antibodies to DNA/RNA metabolism Algal protein extraction buffer Secondary antibodies
Additional information

application information
Recommended dilution		1 : 4000 (WB)
Expected \| apparent MW		76.7 kDa
Confirmed reactivity		Synechocystis sp. PCC6803
Predicted reactivity		Cyanobacteria
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references		to be added when available, antibody released in November 2013.

application example

western blot using anti-EF-G1 antibodies

Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 2 minutes.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

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