EF-G1 | elongation factor G1

Recombinant EF-G1 protein from Synechocystis sp. PCC6803, P28371, Cyanobase: slr1463

Application example

Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T  for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in  for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was  2 minutes.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

AS11 1633 | Anti-EF1A | elongation factor 1-alpha / EF-1-alpha, rabbit antibodies

AS10 678 | Anti-eEF1B-alpha1 an 2 | elongation factor 1B-alpha 1 and 2, rabbit antibodies

AS10 677 | Anti-eEF1B-beta1 and 2 | elongation factor 1-beta1 and 1-beta2, rabbit antibodies

AS07 265 | Anti-eEF1b | elongation factor eEF1b-beta protein, rabbit antibodies

Algal protein extraction buffer

Secondary antibodies

EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.