EF-G1 | elongation factor G1

Name: EF-G1 | elongation factor G1
SKU: AS13 2652
Price: 302 EUR
Availability: InStock

AS13 2652 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Streptococcus pneumoniae, Synechocystis sp. PCC6803

EF-G1 | elongation factor G1 in the group Antibodies Plant/Algal / DNA/RNA/Cell Cycle / Translation at Agrisera AB (Antibodies for research) (AS13 2652)

Product Information

Immunogen

Recombinant EF-G1 protein from Synechocystis sp. PCC6803, P28371, Cyanobase: slr1463

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 50 µl

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 4000 (WB)

Expected | apparent MW 76,7 kDa

Reactivity

Confirmed reactivity Streptococcus pneumoniae, Synechocystis sp. PCC6803

Predicted reactivity Cyanobacteria, Nannochloropsis gaditana
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application example

western blot using anti-EF-G1 antibodies

Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 2 minutes.

Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA

Additional information

Background

EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.

Product citations

Selected references Bárria et al. (2019). Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomes. RNA Biol. 2019 Feb;16(2):211-219. doi: 10.1080/15476286.2018.1564616.

immunogen:	Recombinant EF-G1 protein from Synechocystis sp. PCC6803, P28371, Cyanobase: slr1463
Reconstitution:	For reconstitution add 50 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum
Format:	Lyophilized
Quantity:	50 µl
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 4000 (WB)
calculated \| apparent molecular mass [kDa]:	76,7 kDa
Confirmed reactivity:	Streptococcus pneumoniae, Synechocystis sp. PCC6803
predicted reactivity:	Cyanobacteria, Nannochloropsis gaditana Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
Picture (footer):	Application example Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC6803 extracted with SDS-sample buffer and recombinant EF-G1 (right panel) were separated on 10 % SDS-PAGE and blotted 1h to PVDF. Blots were blocked with 5 % milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 7 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 2 minutes. Courtesy of Yichen Zhang, Department of Biochemistry and Molecular Biology, University of Massachusetts, USA
background:	EF-G1 (elongation factor G1) is catalyzing the GTP-dependent ribosomal translocation step during translation elongation. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome. Portein is located in cytoplasm.
All references:	Bárria et al. (2019). Pneumococcal RNase R globally impacts protein synthesis by regulating the amount of actively translating ribosomes. RNA Biol. 2019 Feb;16(2):211-219. doi: 10.1080/15476286.2018.1564616.