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Anti-Extensin Glycoprotein (monoclonal, clone JIM19)
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
- Product Info
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Sub class: IgM Immunogen: Polysaccharide extensin glycoprotein Host: Rat Clonality: Monoclonal Purity: Cell culture supernatant Format: Liquid Quantity: 1 ml Storage: Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from any material adhering to the cap or sides of the tube. Tested applications: Immunoflourescence (IF), Western blot (WB) Recommended dilution: 1:10 (IF), 1: 50 (WB) - Reactivity
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Confirmed reactivity: Higher plants, ferns and mosses Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Contains 0.05% sodium azide.
Application of this antibody in Western blot analysis is described in the following publication: Xue et al. (2017). Arabidopsis thaliana FLA4 functions as a glycan-stabilized soluble factor via its carboxy-proximal Fasciclin 1 domain. Plant J . 2017 Aug;91(4):613-630. doi: 10.1111/tpj.13591. Epub 2017 Jun 13. - Background
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Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones. - Product Citations
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Selected references: Davies et al. (1997) Induction of extracellular matrix glycoproteins in Brassica petioles by wounding and in response to Xanthomonas campestris. Mol Plant Microbe Interact. 1997;10(7):812-820. doi:10.1094/MPMI.1997.10.7.812
Wang. et al. (1995) The monoclonal antibody JIM19 modulates abscisic acid action in barley aleurone protoplasts. Planta 196, 271-276 (1995). https://doi.org/10.1007/BF00201384 - Reviews:
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From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
