Fer1 | Ferritin 1 (pre-apoferritin)

AS06 126 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii ferritin1 protein sequence Q8LRU1

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 200 µl

Reconstitution For reconstitution add 200 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW

27 (with transit peptide) | 25 for Chlamydomonas reinhardtii

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

A picture with a western blot application sample can be obtained from the reference given below

Background

Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.

Product citations

Selected references Long et al. (2008) Genetics 179: 137-147

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calculated \| apparent molecular mass [kDa]:	27 (with transit peptide) \| 25 for Chlamydomonas reinhardtii
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii ferritin1 protein sequence Q8LRU1
Purity:	Serum
Quantity:	200 µl
recommended dilution:	1 : 1000 (WB)
Reconstitution:	For reconstitution add 200 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Chlamydomonas reinhardtii
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information (application):	A picture with a western blot application sample can be obtained from the reference given below
All references:	Long et al. (2008) Genetics 179: 137-147
background:	Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.