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Tag Antibody Set 

Fer2 | ferrritin 2

AS06 196  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Chlamydomonas reinhardtii

Fer2 | ferrritin 2  in the group Antibodies for Plant/Algal  / Developmental Biology / Ion metabolism at Agrisera AB (Antibodies for research) (AS06 196)

PRODUCT INFORMATION IN PDF

Qty: 
355 €

Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Fer2 protein sequence (A8WCT5) from Chlamydomonas reinhardtii 

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

32 (with transitpeptide) | 16 (monomer) and ~60 (trimer) for Chlamydomonas reinhardtii

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii
Predicted reactivity

Chlamydomonas reinhardtii

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

A picture with a western blot application example can be found in the reference given below.

Related products

Related products

AS06 126  Anti-Fer1 | ferritin 1(pre-apoferritin), rabbit antibodies

Collection of antibodies to Chlamydomonas proteins

Background

Background

Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis.

Product citations

Selected references Long et al. (2008) Genetics 179: 137-147

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