Name:
Phone:
E-mail:
Message


 


FNR | Ferredoxin NADP Reductase (root)

AS20 4436 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thaliana, Triticum aestivum, Secale cereale, Zea mays | Plastidial marker (roots)
FNR | Ferredoxin  NADP Reductase (root) in the group Antibodies for Plant/Algal  / Photosynthesis  / Electron transfer at Agrisera AB (Antibodies for research) (AS20 4436)

DATA SHEET IN PDF

Qty: 
305
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen Purified full length, tag cleaved, recombinant maize root FNR (R-FNR),  UniProt: Q41736

Host Rabbit
Clonality Polyclonal
Purity Total IgG, purified on Protein A
Format Liquid at 1 mg/ml in PBS, 50% glycerol. Filter sterilized. No preservative or carrier added.
Quantity 100 µg
Storage Store at -20°C; once make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1: 1000 - 1: 30 000 (WB)
Expected | apparent MW 36.3 kDa | 35.57 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Triticum aestivum, Secale cereale, Zea mays
Predicted reactivity Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Western blot using anti-FNR antibodies
Secale cereale total chloroplast extract (leaves) (1), Triticum aestivum total chloroplast extract (leaves) (2), Triticum aestivum cytosolic protein extract (roots) (3), Secale cereale cytosolic protein extract (roots) (4),  Triticum aestivum total protein extract (leaves) (5)Secale cereale total protein extract (leaves) (6), Secale cereale total plastid extract (roots) (7)Secale cereale total protein extract (roots) (8)

15 μg of protein extracted from leaves and roots of Triticum aestivum and Secale cereale were separated on 10% SDS-PAGE and blotted 1h onto nitrocellulose (0.45um) using semi-dry transfer. After blocking with 5% milk in TBST, blots were incubated with the primary antibody at a dilution of 1:5000 in TBST for 1h at room temperature. Following incubation and wash steps, blots were incubated with secondary Goat anti-Rabbit IgG (H&L), HRP conjugated (AS09 602, Agrisera) for 1 hour at a dilution of 1:40 000. Blots were developed with the HRP detection system using AgriseraECLSuperBright.


Courtesy of Dr Bartosz Szabała,Department of Plant Genetics, Breeding and Biotechnology, Warsaw University of Life Sciences, Poland


Western blot using anti-root FNR antibodies

10 μg/well of root total protein of Arabidopsis thaliana wild type leaf (1), (3) and mutants rfer1 (2) and rfnr2-2 (4) grown under 0.2 mM nitrate for 7 days were freshly extracted with 2x SDS-sample buffer (+ 2ME) for SDS-PAGE and denatured with 4X SDS buffer at 95°C for 5 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF membrane. Blot was blocked with 3 % skim milk/TBS-T, 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2000 in TBS-T for 1-2h/RT. The antibody solution was decanted and the blot was washed 4 times for 10 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in for 1h/RT with agitation. The blot was washed as above and developed with a chemiluminescent detection reagent, following manufacture's recommendation.

R-FNR2 is a dominant form in wild type roots.

Mutant rfnr1 produces R-FNR2 and mutant rfr2 produces R-FNR1.

Additional information

Antibody will recognize two root FNR proteins (R-FNR1 and R-FNR2) of Arabidopsis thaliana and Zea mays as well as leaf FNRs.

This antibody can be used as a marker of plastids localised in roots.

Related products

Related products AS20 4439 | Anti-FNR1 | Ferredoxin NADP Reductase, isoprotein 1 (leaf), rabbit antibodies
AS20 4438 | Anti-FNR2 | Ferredoxin NADP Reductase, isoprotein 2 (leaf), rabbit antibodies
AS20 4437 | Anti-FNR3 | Ferredoxin NADP Reductase, isoprotein 3 (leaf), rabbit antibodies

collection of antibodies to proteins involved in photosynthesis

Background

Background Ferredoxin-NADP reductase (FNR) isoproteins of plant roots play a key role in redox homeostasis of NADPH / NADP + and donation of reducing equivalence to ferredoxin. R-FNR2 is major form of R-FNR and involved in reduction and detoxication of nitrite in root.

Product citations

Selected references Hachiya et al. (2016). Arabidopsis Root-Type Ferredoxin:NADP(H) Oxidoreductase 2 Is Involved in Detoxification of Nitrite in Roots . Plant Cell Physiol. 57(11):2440-2450. doi: 10.1093/pcp/pcw158.
Hachiya et al. (2016). Arabidopsis Root-Type Ferredoxin:NADP(H) Oxidoreductase 2 Is Involved in Detoxification of Nitrite in Roots. Plant Cell Physiol. 57(11):2440-2450. doi: 10.1093/pcp/pcw158.
Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 123(3):1037-45. doi: 10.1104/pp.123.3.1037.
Onda et al. (2000). Differential Interaction of Maize Root ferredoxin:NADP(+) Oxidoreductase With Photosynthetic and Non-Photosynthetic Ferredoxin Isoproteins. Plant Physiol. 123(3):1037-45. doi: 10.1104/pp.123.3.1037.

Related products: FNR | Ferredoxin NADP Reductase (root)

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection
From 15 €
AS20 4439 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
330 €
AS20 4437 | Clonality: Polyclonal  |  Host: Rabbit |  Reactivity: Arabidopsis thali...
305 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
190 €