ALD | Fructose-1,6 bisphosphate aldolase

AS08 294 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, C. arietinum, E. tef, G. gracilis, O. sativa, P. falciparum, T. salsuginea

ALD | Fructose-1,6 bisphosphate aldolase

Product Information

Immunogen

overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930

Host Rabbit

Clonality Polyclonal

Purity Serum

Format Lyophilized

Quantity 100 µl

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 5000 (WB)

Expected | apparent MW

38 | 38 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica napus, Cicer arietinum, Eragrostis tef, Gracilaria gracilis (red algae), Oryza sativa, Plasmodium chabaudi, Plasmodium falciparum, Thellungiella salsuginea

Predicted reactivity Glycne max, Nicotiana attenuata, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Physcomitrium patens, Pisum sativum, Populus jackii, Spinacia oleracea, Vitis vinifera, Zea mays
Species of your interest not listed? Contact us

Not reactive in

Synechocystis sp.

Application examples

Reactant: Oryza sativa (Asian rice)

Application: Western Blotting

Pudmed ID: 26552588

Journal: Sci Rep

Figure Number: 6A

Published Date: 2015-11-10

First Author: Dixit, G., Singh, A. P., et al.

Impact Factor: 4.13

Open Publication

Western blot analysis of selected candidate proteins (AtpB, 2Fe-2S, GLN2, Psb R, PRK, ALD) with their corresponding molecular weight in rice leaves during As stress under various S regimes (A); RuBisCo large subunit a band in coomassie blue staining (CBB) SDS gel served as a loading control (B), which used for normalization of detected proteins in densitometry studies (C) fold change indicated with respect of control samples.

Reactant: Cicer arietinum (Chickpea)

Application: Western Blotting

Pudmed ID: 27503257

Journal: Sci Rep

Figure Number: 7C

Published Date: 2016-08-09

First Author: Parveen, S., Gupta, D. B., et al.

Impact Factor: 4.13

Open Publication

Compartmentalization of CaFer1.(A) Bioinformatics prediction of CaFer1 to the chloroplast and the secretome. (B) The model of CaFer1 generated by comparative modeling, shown in ribbon view (right upper panel). The percent identity of modeled structure of CaFer1 with the template, and the RMSD values were given in the table (right lower panel). The non-conserved amino acid sequence of CaFer1 (red color) from that of other phytoferritins with predicted signal peptide, is shown in left panel. (C) Purity assessment and immunodetection of CaFer1, FBA and XET in the chickpea secretome. Immunoreactions with Rubisco and GAPDH served as negative controls for secretome. (D) BFA-mediated inhibition of CaFer1 and thioredoxin transport. ‘?BFA’ represents the untreated secretome. (E) CaFer1 was detected in extracellular space and chloroplast of OE plants with anti-FLAG antibody. XET was used as positive control for extracellular space and rubisco for chloroplast. WT, wild-type; OE, CaFer1-overexpressing.

Additional information

This product can be sold containing ProClin if requested

Background

Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.

Product citations

Selected references Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.
Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.
Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.
Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.
Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.

background:	Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
calculated \| apparent molecular mass [kDa]:	38 \| 38 kDa
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930
Purity:	Serum
Quantity:	100 µl
recommended dilution:	1 : 5000 (WB)
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
More images:	Reactant: Oryza sativa (Asian rice) Application: Western Blotting Pudmed ID: 26552588 Journal: Sci Rep Figure Number: 6A Published Date: 2015-11-10 First Author: Dixit, G., Singh, A. P., et al. Impact Factor: 4.13 Open Publication Western blot analysis of selected candidate proteins (AtpB, 2Fe-2S, GLN2, Psb R, PRK, ALD) with their corresponding molecular weight in rice leaves during As stress under various S regimes (A); RuBisCo large subunit a band in coomassie blue staining (CBB) SDS gel served as a loading control (B), which used for normalization of detected proteins in densitometry studies (C) fold change indicated with respect of control samples. Reactant: Cicer arietinum (Chickpea) Application: Western Blotting Pudmed ID: 27503257 Journal: Sci Rep Figure Number: 7C Published Date: 2016-08-09 First Author: Parveen, S., Gupta, D. B., et al. Impact Factor: 4.13 Open Publication Compartmentalization of CaFer1.(A) Bioinformatics prediction of CaFer1 to the chloroplast and the secretome. (B) The model of CaFer1 generated by comparative modeling, shown in ribbon view (right upper panel). The percent identity of modeled structure of CaFer1 with the template, and the RMSD values were given in the table (right lower panel). The non-conserved amino acid sequence of CaFer1 (red color) from that of other phytoferritins with predicted signal peptide, is shown in left panel. (C) Purity assessment and immunodetection of CaFer1, FBA and XET in the chickpea secretome. Immunoreactions with Rubisco and GAPDH served as negative controls for secretome. (D) BFA-mediated inhibition of CaFer1 and thioredoxin transport. ‘?BFA’ represents the untreated secretome. (E) CaFer1 was detected in extracellular space and chloroplast of OE plants with anti-FLAG antibody. XET was used as positive control for extracellular space and rubisco for chloroplast. WT, wild-type; OE, CaFer1-overexpressing.
Confirmed reactivity:	Arabidopsis thaliana, Brassica napus, Cicer arietinum, Eragrostis tef, Gracilaria gracilis (red algae), Oryza sativa, Plasmodium chabaudi, Plasmodium falciparum, Thellungiella salsuginea
predicted reactivity:	Glycne max, Nicotiana attenuata, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Physcomitrium patens, Pisum sativum, Populus jackii, Spinacia oleracea, Vitis vinifera, Zea mays Species of your interest not listed? Contact us
not reactive in:	Synechocystis sp.
All references:	Wang et al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2. Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032. Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018. Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218. Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449. Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205. Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
additional information (application):	This product can be sold containing ProClin if requested