ALD | Fructose-1,6 bisphosphate aldolase
AS08 294 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. napus, C. arietinum, E. tef, G. gracilis, O. sativa, P. falciparum, T. salsuginea

Data sheet | Product citations | Protocols | Customer reviews |
Product Information
overexpressed cytosolic fructose 1,6 bisphosphate aldolase (ALD) based on the sequence from Arabidopsis thaliana Q9LF98, At3g52930
38 | 38 kDa
Reactivity
Species of your interest not listed? Contact us
Synechocystis sp.
Application examples

Reactant: Oryza sativa (Asian rice)
Application: Western Blotting
Pudmed ID: 26552588
Journal: Sci Rep
Figure Number: 6A
Published Date: 2015-11-10
First Author: Dixit, G., Singh, A. P., et al.
Impact Factor: 4.13
Open PublicationWestern blot analysis of selected candidate proteins (AtpB, 2Fe-2S, GLN2, Psb R, PRK, ALD) with their corresponding molecular weight in rice leaves during As stress under various S regimes (A); RuBisCo large subunit a band in coomassie blue staining (CBB) SDS gel served as a loading control (B), which used for normalization of detected proteins in densitometry studies (C) fold change indicated with respect of control samples.

Reactant: Cicer arietinum (Chickpea)
Application: Western Blotting
Pudmed ID: 27503257
Journal: Sci Rep
Figure Number: 7C
Published Date: 2016-08-09
First Author: Parveen, S., Gupta, D. B., et al.
Impact Factor: 4.13
Open PublicationCompartmentalization of CaFer1.(A) Bioinformatics prediction of CaFer1 to the chloroplast and the secretome. (B) The model of CaFer1 generated by comparative modeling, shown in ribbon view (right upper panel). The percent identity of modeled structure of CaFer1 with the template, and the RMSD values were given in the table (right lower panel). The non-conserved amino acid sequence of CaFer1 (red color) from that of other phytoferritins with predicted signal peptide, is shown in left panel. (C) Purity assessment and immunodetection of CaFer1, FBA and XET in the chickpea secretome. Immunoreactions with Rubisco and GAPDH served as negative controls for secretome. (D) BFA-mediated inhibition of CaFer1 and thioredoxin transport. ‘?BFA’ represents the untreated secretome. (E) CaFer1 was detected in extracellular space and chloroplast of OE plants with anti-FLAG antibody. XET was used as positive control for extracellular space and rubisco for chloroplast. WT, wild-type; OE, CaFer1-overexpressing.
Additional information
Background
Fructose-1,6 bisphosphate aldolase (ALD) is an enzyme catalazying a key reaction of glycolysis and energy production, converting D-fructose- 1,6-bisphospate into dihydroxyacetone phosphate and D-glyceraldehyde-3-phosphate. This enzyme is present in plant and animal tissues. Plant enzyme is a class I aldolase which does not require a bivalent metal cofactor. It is located to outer mitochondrial membrane.
Product citations
Kamies et al. (2017). A Proteomic Approach to Investigate the Drought Response in the Orphan Crop Eragrostis tef. Proteomes. 2017 Nov 15;5(4). pii: E32. doi: 10.3390/proteomes5040032.
Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
Parveen et al. (2016). Chickpea Ferritin CaFer1 Participates in Oxidative Stress Response, and Promotes Growth and Development. Sci Rep. 2016 Aug 9;6:31218. doi: 10.1038/srep31218.
Yam et al. (2016). Characterization of the Plasmodium Interspersed Repeats (PIR) proteins of Plasmodium chabaudi indicates functional diversity. Sci Rep. 2016 Mar 21;6:23449. doi: 10.1038/srep23449.
Dixit (2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.
Vera-Estrella et al. (2014). Comparative 2D-DIGE analysis of salinity responsive microsomal proteins from leaves of salt-sensitive Arabidopsis thaliana and salt-tolerant Thellungiella salsuginea. J Proteomics. 2014 Jun 2. pii: S1874-3919(14)00288-7. doi: 10.1016/j.jprot.2014.05.018.
Related products: ALD | Fructose-1,6 bisphosphate aldolase
This product can b...
10 % until...
10 ...