G4 | Chlorophyll synthase (chloroplastic)
AS14 2793 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Hordeum vulgare

Data sheet | Product citations | Add review |
Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana chlorophyll synthase, UniProt: Q38833, TAIR: AT3G51820
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 µl
Reconstitution
For reconstitution add 50 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
41.8 kDa (further processed into a mature form)
Reactivity
Confirmed reactivity
Hordeum vulgare
Predicted reactivity
Arabidopsis thaliana, Avena sativa, Camelia sinensis, Cocomyxa subellipsoidea, Gossypium hirsutum, Micromonas pusilla, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Ricinus communis, Triticum urartu, cyanobacteria
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
application example

Barley seeds were grown for 4.5 days in the dark before they were harvested and intact plastids were isolated in light according to Eichacker et al. 1996. The concentration of plastids was determined by counting in a Thoma counting chamber. The membrane complexes were isolated according to Reisinger et al. 2008 before 3x SB buffer (2% (w/v) SDS, 10% (w/v) sucrose, 0.03% (w/v) bromphenol blue, 66 mM NaCO3, 66 mM dithiothreitol) was added. The proteins were denatured for 2 min at 72 °C. The membrane fractions were loaded onto a 12.5 % SDS-acrylamide gel, giving approximately 2.5x107 plastids per lane alongside with Magic Marc (LC5602, Life technologies). The gel was then run at 175 V with 1xSDS running buffer. The proteins were then transferred onto a nitrocellulose membrane by using semi-wet transfer and Towbin buffer w/10 % methanol. The proteins were transferred at 2 mA/cm2 for 1 hour. The membrane was blocked with TBS-milk, washed 3x5 min in TBS-tween and the primary antibody (Chlorophyll synthase, AS14 2793 from Agrisera) was added at a concentration of 1:1000. The membrane was incubated ON at 4 °C (approximately 18 h) before washing with TBS-tween and incubation for 1h at RT with the secondary antibody diluted 1:12 500 (Agrisera AS09 602). After washing, the membrane was incubated in an ECL1/ECL2 solution (100 mM Tris, 2.5 mM Luminol, 0.4 mM pCoumaracid/100 mM Tris, 0.0183 % Hydrogenperoxide) before exposure to a film for 7 min. The film was then developed and fixed using chemicals from Kodak.
Courtesy of Eli Drange Vee, Staff Engineer, Centre for Organelle Research, University of Stavanger, Norway

Barley seeds were grown for 4.5 days in the dark before they were harvested and intact plastids were isolated in light according to Eichacker et al. 1996. The concentration of plastids was determined by counting in a Thoma counting chamber. The membrane complexes were isolated according to Reisinger et al. 2008 before 3x SB buffer (2% (w/v) SDS, 10% (w/v) sucrose, 0.03% (w/v) bromphenol blue, 66 mM NaCO3, 66 mM dithiothreitol) was added. The proteins were denatured for 2 min at 72 °C. The membrane fractions were loaded onto a 12.5 % SDS-acrylamide gel, giving approximately 2.5x107 plastids per lane alongside with Magic Marc (LC5602, Life technologies). The gel was then run at 175 V with 1xSDS running buffer. The proteins were then transferred onto a nitrocellulose membrane by using semi-wet transfer and Towbin buffer w/10 % methanol. The proteins were transferred at 2 mA/cm2 for 1 hour. The membrane was blocked with TBS-milk, washed 3x5 min in TBS-tween and the primary antibody (Chlorophyll synthase, AS14 2793 from Agrisera) was added at a concentration of 1:1000. The membrane was incubated ON at 4 °C (approximately 18 h) before washing with TBS-tween and incubation for 1h at RT with the secondary antibody diluted 1:12 500 (Agrisera AS09 602). After washing, the membrane was incubated in an ECL1/ECL2 solution (100 mM Tris, 2.5 mM Luminol, 0.4 mM pCoumaracid/100 mM Tris, 0.0183 % Hydrogenperoxide) before exposure to a film for 7 min. The film was then developed and fixed using chemicals from Kodak.
Courtesy of Eli Drange Vee, Staff Engineer, Centre for Organelle Research, University of Stavanger, Norway
Additional information
Background
Background
G4 (Chlorophyll synthase) is an enzyme which performs estrification of chlorophyllide (a and b), which is the last step of chlorophyll biosynthesis. Alternative names: polyprenyl transferase, protein G4.
Product citations
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