NtrC | Thioredoxin reductase (TR/TRxR) (higher plants)

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

55.3 kDa (Chlamydomonas reinhardtii), 57.9 kDa (Arabidopsis thaliana), further processed into the matured form

Anthurium amnicola, Brachypodium distachyon, Brassica napus, Brassica oleracea, Capsella rubella, Capsicum annuum, Cephalotus follicularis, Chlamydomonas reinhardtii, Chlamydia sp., Cicer arietinum, Citrus sinensis, Cucumis melo, Cucumis sativus, Dendrobium catenatum, Eutrema salsugineum, Genlisea aurea, Ostreococcus luminarius, Synechococcus sp., Glycine soja, Glycine max, Gossypium raimondii, Hordeum vulgare, Juglans regia, Leersia perrieri, Manihot esculenta, Medicago truncatula, Musa acuminata subsp. malaccensis, Nelumbo nucifera, Noccaea caerulescens, Oryza barthii, Phoenix dactylifera, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zea mays, Vigna angularis var. angularis, Vitis vinifera

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

Collection of antibodies to Chlamydomonas proteins

Algal protein extraction buffer

Secondary antibodies

Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx) Involved in chloroplast protection against oxidative damage. Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis thaliana, which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein   size of ca. 488 amino acids.