Anti-NtrC | Thioredoxin reductase (TR/TRxR) (higher plants)

Product no: AS07 243

AS07 243 |  Clonality: Polyclonal | Host: Rabbit  | Reactivity: Higher plants

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  • Product Info
  • Immunogen:

    KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

    The peptide is perfectly conserved in Arabidopsis thaliana NTRC, UniProt: , TAIR: AT2G41680
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 200 g
    Reconstitution: For reconstitution add 300 l of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 500 (WB)
    Expected | apparent MW:

    55.3 kDa (Chlamydomonas reinhardtii), 57.9 kDa (Arabidopsis thaliana), further processed into the matured form

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity:

    Anthurium amnicola, Brachypodium distachyon, Brassica napus, Brassica oleracea, Capsella rubella, Capsicum annuum, Cephalotus follicularis, Chlamydomonas reinhardtii, Chlamydia sp., Cicer arietinum, Citrus sinensis, Cucumis melo, Cucumis sativus, Dendrobium catenatum, Eutrema salsugineum, Genlisea aurea, Ostreococcus luminarius, Synechococcus sp., Glycine soja, Glycine max, Gossypium raimondii, Hordeum vulgare, Juglans regia, Leersia perrieri, Manihot esculenta, Medicago truncatula, Musa acuminata subsp. malaccensis, Nicotiana tabacum, Nelumbo nucifera, Noccaea caerulescens, Oryza barthii, Phoenix dactylifera, Populus trichocarpa, Ricinus communis, Solanum lycopersicum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zea mays, Vigna angularis var. angularis, Vitis vinifera

    Species of your interest not listed? Contact us
  • Additional Information
  • Additional information:

    Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

  • Background
  • Background:

    Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC which is reducing thoredoxin (Trx) Involved in chloroplast protection against oxidative damage. Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis thaliana, which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein   size of ca. 488 amino acids.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts
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