NtrC | Thioredoxin reductase (TR/TRxR) (algal)

Name: NtrC | Thioredoxin reductase (TR/TRxR) (algal)
SKU: AS06 159
Price: 302 EUR
Availability: InStock
Rating: 5 (1 reviews)

AS06 159 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chlamydomonas reinhardtii

NtrC | Thioredoxin reductase (TR/TRxR) (algal) in the group Antibodies Plant/Algal / Chlamydomonas reinhardtii at Agrisera AB (Antibodies for research) (AS06 159)

NtrC | Thioredoxin reductase (TR/TRxR) (algal)

Product Information

Immunogen

KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7

Host Rabbit

Clonality Polyclonal

Purity Immunogen affinity purified serum in PBS pH 7.4.

Format Lyophilized

Quantity 200 µg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 1 : 500 (WB)

Expected | apparent MW 55,3 | 49 kDa (following processing)

Reactivity

Confirmed reactivity Chlamydomonas reinhardtii

Predicted reactivity

Chlamydia sp., Marchantia polymorpha, Nannochloropsis gaditana, Ostreococcus luminarius, Physcomitrium patens, Synechococcus sp.

Species of your interest not listed? Contact us

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria

Antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.

Background

Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.

Product citations

immunogen:	KLH-conjugated peptide derived from N-terminal domain of NtrC of Chlamydomonas reinhardtii A8HNQ7
Reconstitution:	For reconstitution add 100 µl of sterile water
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Immunogen affinity purified serum in PBS pH 7.4.
Format:	Lyophilized
Quantity:	200 µg
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	1 : 500 (WB)
calculated \| apparent molecular mass [kDa]:	55,3 \| 49 kDa (following processing)
Confirmed reactivity:	Chlamydomonas reinhardtii
predicted reactivity:	Chlamydia sp., Marchantia polymorpha, Nannochloropsis gaditana, Ostreococcus luminarius, Physcomitrium patens, Synechococcus sp. Species of your interest not listed? Contact us
not reactive in:	Arabidopsis thaliana
additional information:	Peptide target chosen from the N-terminal domain, nearly fully conserved within 3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteins from Chlamydomonas reinhardtii and Synechococcus sp. 7942 and other cyanobacteria
additional information (application):	Antibody will react with long NtrC of Chlamydomonas reinhardtii proteins and shorter NtrA-type rpoteins, which would be distinguished by migration size.
background:	Thioredoxin Reductase (TR, TrxR) is the only known enzyme (EC 1.8.1.9) which is reducing thoredoxin (Trx). Activity of thoredoxin is essential for growth and survival of the cell. There seem to be one isoform of NtrC protein in Arabidopsis which includes an N-terminal reductase domain and a C-terminal domain related to thioredoxin proteins. Arabidopsis thaliana and Chlmydomonas reinhardtii NtrC proteins are ca. 568 amino acids long, but include ca. 80 amino acid signal peptides, for mature protein size of ca. 488 amino acids.