GDP-L-Galactose Phosphorylase

AS10 723 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea var. italica, B. rapa var. komatsuna, C. limon, N. tabacum, S. oleracea, Z. mays, Ch. reinhardtii

GDP-L-Galactose Phosphorylase in the group Antibodies Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 723)
GDP-L-Galactose Phosphorylase


How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information


KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 1200 , 1 : 3000 (WB)
Expected | apparent MW

51 | 50 kDa


Confirmed reactivity Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Predicted reactivity Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patens
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

application example


western blot using VTC2 antibodies

9 ul of total soluble protein from Arabidopsis thaliana (1), Zea mays (2), Nicotiana tabacum (3), Citrus lemon (4), Spinacia oleracea (5), Brassica rapa var. komatsuna (6), Brassica oleracea var. italica (7) was separated on 12% SDS-PAGE and blotted 1.5h to PVDF at 1.5 mA/cm2 constant current. Blots were blocked immediately following transfer in TBS-0.3% Tween 20 + 5% dried milk overnight at room temperature (RT) with agitation. Blots were incubated in the primary antibody AS10 723 at a dilution of 1: 1500 for 4 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once with TBS-T+5%milk, followed by washing 3 times for 5 min in the same at RT with agitation. Blots were incubated in secondary antibodies (goat anti-rabbit IgG HRP conjugated from Biorad) diluted in TBS-T+5%milk to 1:25 000 for 1 h at RT with agitation. The blots were washed 2 x 5 mins with TBS-T+5% milk as above, then rinsed with TBS and followed by ECL detection (approx 5 minutes).

Courtesy Dr. Eugen Urzica, UCLA

Additional information

Related products



GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.

Product citations

Related products: GDP-L-Galactose Phosphorylase

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

This product can b...
From 25 €
AS09 607 |  Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
199 €
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
194 €