AS10 723 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea var. italica, B. rapa var. komatsuna, C. limon, N. tabacum, S. oleracea, Z. mays, Ch. reinhardtii
- Product Info
Immunogen: Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 200 µl Reconstitution: For reconstitution add 200 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 1200 , 1 : 3000 (WB) Expected | apparent MW:
51 | 50 kDa
Confirmed reactivity: Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii Predicted reactivity: Manihot esculenta, Sorghum bicolor, Oryza sativa, Physcomitrium patens
Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
- Application Examples
9 ul of total soluble protein from Arabidopsis thaliana (1), Zea mays (2), Nicotiana tabacum (3), Citrus lemon (4), Spinacia oleracea (5), Brassica rapa var. komatsuna (6),Brassica oleracea var. italica (7) was separated on 12% SDS-PAGE and blotted 1.5h to PVDF at 1.5 mA/cm2 constant current. Blots were blocked immediately following transfer in TBS-0.3% Tween 20 + 5% dried milk overnight at room temperature (RT) with agitation. Blots were incubated in the primary antibody AS10 723 at a dilution of 1: 1500 for 4 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once with TBS-T+5%milk, followed by washing 3 times for 5 min in the same at RT with agitation. Blots were incubated in secondary antibodies (goat anti-rabbit IgG HRP conjugated from Biorad) diluted in TBS-T+5%milk to 1:25 000 for 1 h at RT with agitation. The blots were washed 2 x 5 mins with TBS-T+5% milk as above, then rinsed with TBS and followed by ECL detection (approx 5 minutes).
Courtesy Dr. Eugen Urzica, UCLA
GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.
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AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
10 % until end of 2023. Use discount code: Conj10
Now available only as separate products:
1 x AS16 ECL-N
1 x AS16 ECL-S
This product can be purchased in 3 different volumes:
AS16 ECL-SN-10, 10 ml. Trial size limited to one per customer
AS16 ECL-SN, 100 ml
AS16 ECL-SN-1L, 1L
Choose the appropriate volume in the drop down menu to the right