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Tag Antibody Set 

GDP-L-Galactose Phosphorylase

AS10 723 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, B. oleracea var. italica, B. rapa var. komatsuna, C. limon, N. tabacum, S. oleracea, Z. mays, Ch. reinhardtii

GDP-L-Galactose Phosphorylase in the group Antibodies for Plant/Algal  / Environmental Stress / Oxidative stress at Agrisera AB (Antibodies for research) (AS10 723)

PRODUCT INFORMATION IN PDF

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Datasheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjuated synthetic peptide derived from known GDP-L-Galactose Phosphorylase sequences, including Arabidopsis thaliana Q8LKQ7 (At4g26850) and Chlamydomonas reinhardtii

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1200 , 1 : 3000 (WB)
Expected | apparent MW

51 | 50 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Brassica oleracea var. italica, Brassica rapa var. komatsuna, Citrus limon, Nicotiana tabacum, Spinacia oleracea, Zea mays, Chlamydomonas reinhardtii
Predicted reactivity Sorghum bicolor, Oryza sativa, Physcomitrella patens
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples

application example

 

western blot using VTC2 antibodies

9 ul of total soluble protein from Arabidopsis thaliana (1), Zea mays (2), Nicotiana tabacum (3), Citrus lemon (4), Spinacia oleracea (5), Brassica rapa var. komatsuna (6), Brassica oleracea var. italica (7) was separated on 12% SDS-PAGE and blotted 1.5h to PVDF at 1.5 mA/cm2 constant current. Blots were blocked immediately following transfer in TBS-0.3% Tween 20 + 5% dried milk overnight at room temperature (RT) with agitation. Blots were incubated in the primary antibody AS10 723 at a dilution of 1: 1500 for 4 h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly once with TBS-T+5%milk, followed by washing 3 times for 5 min in the same at RT with agitation. Blots were incubated in secondary antibodies (goat anti-rabbit IgG HRP conjugated from Biorad) diluted in TBS-T+5%milk to 1:25 000 for 1 h at RT with agitation. The blots were washed 2 x 5 mins with TBS-T+5% milk as above, then rinsed with TBS and followed by ECL detection (approx 5 minutes).

Courtesy Dr. Eugen Urzica, UCLA

Additional information

Related products

Background

Background

GDP-L-Galactose Phosphorylase is a histidine triad (HIT) enzyme of the Smirnoff-Wheeler pathway of ascorbic acid synthesis in plants. Encoded by VTC2 gene. The enzyme catalyzes the conversion of GDP-L-galactose to L-galactose 1-phosphate in a reaction that consumes inorganic phosphate and produces GDP.

Product citations

Selected references

to be added when available, antibody available in July 2010