EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants

Product no: AS20 4512

AS20 4512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: FP, YFP, EGFP | Green Fluorescence Protein and its variants

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  • Product Info
  • Immunogen: Recombinant, His-tagged EGFP protein from Aequorea victoria, UniProt: P42212
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum. Contains 0.05 % sodium azide.
    Format: Liquid
    Quantity: 100 l
    Storage: Store at -20C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
    Recommended dilution: assay dependent (IP), 1 : 4000 (IF), 1 : 2000 (WB)
  • Reactivity
  • Confirmed reactivity: EGFP, S65T-GFP, RS-GFP, YFP
  • Application Examples
  • Western blot using anti-GFP antibodies

    Lysate of 293T celss trasfected with empty vector (letf panel "-")
    Lysate of 293T cells transfected with the plasmid carrying the GFP gene

    Membrane for transfer: nitrocellulose. 
    Anti-GFP antibodies were used at 1: 2000 dilution 1h/RT incubation in TBS-T. Followed by washes and incubation with secondary antibodies, HRP-conjugated and detection using chemiluminescence, following manufacture's recommendations. 

    Immunoprecipitation using anti-GFP antibodies

    -: Lysate of 293T cells transfected with an empty vector
    +: Lysate of 293T cells transfected with carrying the GFP-tagged NPM1 gene

    Immunofluorescence using anti-GFP antibodies

    Immunofluorescence of GFP- and G196-tagged nuclear protein in fission yeast Atf1-G196-GFP expressing cells. Fixation: 3 % formaldehyde. 
    Anti-GFP polyclonal antibodies: 1: 1000 over night incubation at RT, followed by incubation with Alexa-488 anti-rabbit secondary antibodies. 

  • Background
  • Background:

    GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

  • Product Citations
  • Selected references: Tatsumi et al. (2017). G196 epitope tag system: a novel monoclonal antibody, G196,recognizes the small, soluble peptide DLVPR with high affinity. Sci Rep. 2017 Mar 7;7:43480.doi: 10.1038/srep43480. (Immunofluorescence, Western blot)
  • Protocols
  • Antibody protocols
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