EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants
AS20 4512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: FP, YFP, EGFP | Green Fluorescence Protein and its variants
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Lysate of 293T celss trasfected with empty vector (letf panel "-")
Lysate of 293T cells transfected with the plasmid carrying the GFP gene
Membrane for transfer: nitrocellulose.
Anti-GFP antibodies were used at 1: 2000 dilution 1h/RT incubation in TBS-T. Followed by washes and incubation with secondary antibodies, HRP-conjugated and detection using chemiluminescence, following manufacture's recommendations.
-: Lysate of 293T cells transfected with an empty vector
+: Lysate of 293T cells transfected with carrying the GFP-tagged NPM1 gene
Immunofluorescence of GFP- and G196-tagged nuclear protein in fission yeast Atf1-G196-GFP expressing cells. Fixation: 3 % formaldehyde.
Anti-GFP polyclonal antibodies: 1: 1000 over night incubation at RT, followed by incubation with Alexa-488 anti-rabbit secondary antibodies.
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
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