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EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants

AS20 4512 | Clonality: Polyclonal | Host: Rabbit | Reactivity: FP, YFP, EGFP | Green Fluorescence Protein and its variants

EGFP, S65T-GFP, RS-GFP, YFP| Green Fluorescence Protein and its variants in the group Tag Antibodies / GFP/YFP/RFP/CFP at Agrisera AB (Antibodies for research) (AS20 4512)

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Data sheet Product citations Protocols Add review

Product Information

Immunogen Recombinant, His-tagged EGFP protein from Aequorea victoria, UniProt: P42212
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Liquid with 0.05 % sodium azide
Quantity 100 µl
Storage Store at -20°C. Avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution assay dependent (IP), 1 : 4000 (IF), 1 : 2000 (WB)

Reactivity

Confirmed reactivity EGFP, S65T-GFP, RS-GFP, YFP

Application examples

Application examples Western blot using anti-GFP antibodies

Lysate of 293T celss trasfected with empty vector (letf panel "-")
Lysate of 293T cells transfected with the plasmid carrying the GFP gene

Membrane for transfer: nitrocellulose. 
Anti-GFP antibodies were used at 1: 2000 dilution 1h/RT incubation in TBS-T. Followed by washes and incubation with secondary antibodies, HRP-conjugated and detection using chemiluminescence, following manufacture's recommendations. 

Immunoprecipitation using anti-GFP antibodies

-: Lysate of 293T cells transfected with an empty vector
+: Lysate of 293T cells transfected with carrying the GFP-tagged NPM1 gene

Immunofluorescence using anti-GFP antibodies


Immunofluorescence of GFP- and G196-tagged nuclear protein in fission yeast Atf1-G196-GFP expressing cells. Fixation: 3 % formaldehyde. 
Anti-GFP polyclonal antibodies: 1: 1000 over night incubation at RT, followed by incubation with Alexa-488 anti-rabbit secondary antibodies. 

Additional information

Related products

Related products AS15 3000 | Anti-GFP (protein A purified), rabbit antibodies
AS15 2999 | Anti-GFP (total IgG), rabbit antibodies
AS15 3001 | Anti-GFP, peroxidase conjugated, rabbit antibodies
AS15 2998 | Anti-GFP (total IgY), chicken antibodies
AS15 3002 | GFP (purified recombinant protein)
other tag antibodies

Background

Background

GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.

Product citations

Selected references Tatsumi et al. (2017). G196 epitope tag system: a novel monoclonal antibody, G196,recognizes the small, soluble peptide DLVPR with high affinity. Sci Rep. 2017 Mar 7;7:43480.doi: 10.1038/srep43480. (Immunofluorescence, Western blot)

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