PR-2 | GLU I | Class I beta-1,3-glucanase

Name: PR-2 | GLU I | Class I beta-1,3-glucanase
SKU: AS07 208
Price: 394 EUR
Availability: InStock
Rating: 5 (2 reviews)

AS07 208 | Clonality: Polyclonal | Host: Rabbit | Reactivity: N. benthamiana, N. clevilandii, N. tabacum, Phalenopsis, P.abies, S.lycopersicum, S. tuberosum, V. vinifera | compartment marker of vacuolar contents

PR-2 | GLU I | Class I beta-1,3-glucanase in the group Antibodies Plant/Algal / Environmental Stress / Pathogen attack at Agrisera AB (Antibodies for research) (AS07 208)

PR-2 | GLU I | Class I beta-1,3-glucanase

Product Information

Immunogen

Purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

Host Rabbit

Clonality Polyclonal

Purity Total IgG in PBS pH 7.4. (without Ca++).

Format Lyophilized

Quantity 2 mg

Reconstitution For reconstitution add 100 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunolocalization (IL), Western blot (WB)

Recommended dilution 8 µg/ml (WB)

Expected | apparent MW

37 | 33 kDa

Reactivity

Confirmed reactivity Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Predicted reactivity Dicots, Oryza sativa, Prunus persica

Species of your interest not listed? Contact us

Not reactive in

Arabidopsis thaliana

Application examples

Application example

western blot detection of tobacco class I beta 1,3 glucanase

Detection of tobacco tobacco class I ß 1,3 – glucanase in ng loaded per respective well using anti- tobacco class I ß 1,3 – glucanase antibodies. Primary antibodies have been used at 8 µg/ml.

Additional information

For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51.

This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.

This antibody is purified by affinity chromarography on Portein G.

Background

Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

Product citations

Selected references Li et al. (2021) Penicillium chrysogenum polypeptide extract protects Nicotiana benthamiana against TMV infection through modulation of ABA biosynthesis and callose priming. J Exp Bot. 2021 Mar 4:erab102. doi: 10.1093/jxb/erab102. Epub ahead of print. PMID: 33687058. (Immunolocalization)
Colman et al. (2019). Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry. Volume 143, October 2019, Pages 203-211.
Martin-Saladana et al. (2018). Salicylic acid loaded chitosan microparticles applied to lettuce seedlings: Recycling shrimp fishing industry waste. Carbohydrate Polymers Volume 200, 15 November 2018, Pages 321-331.
Wang et al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201.
Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.
Munger et al. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.

immunogen:	Purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Total IgG in PBS pH 7.4. (without Ca++).
Format:	Lyophilized
Quantity:	2 mg
Reconstitution:	For reconstitution add 100 µl of sterile water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications:	Immunolocalization (IL), Western blot (WB)
recommended dilution:	8 µg/ml (WB)
Expected \| apparent MW:	37 \| 33 kDa
Confirmed reactivity:	Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
predicted reactivity:	Dicots, Oryza sativa, Prunus persica Species of your interest not listed? Contact us
not reactive in:	Arabidopsis thaliana
Picture (footer):	Application example Detection of tobacco tobacco class I ß 1,3 – glucanase in ng loaded per respective well using anti- tobacco class I ß 1,3 – glucanase antibodies. Primary antibodies have been used at 8 µg/ml.
additional information:	For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51. This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.
additional information (application):	Important note: for blocking 5 % skim milk in PBS without Ca++ should be used. This antibody is purified by affinity chromarography on Portein G.
background:	Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase
All references:	Li et al. (2021) Penicillium chrysogenum polypeptide extract protects Nicotiana benthamiana against TMV infection through modulation of ABA biosynthesis and callose priming. J Exp Bot. 2021 Mar 4:erab102. doi: 10.1093/jxb/erab102. Epub ahead of print. PMID: 33687058. (Immunolocalization) Colman et al. (2019). Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry. Volume 143, October 2019, Pages 203-211. Martin-Saladana et al. (2018). Salicylic acid loaded chitosan microparticles applied to lettuce seedlings: Recycling shrimp fishing industry waste. Carbohydrate Polymers Volume 200, 15 November 2018, Pages 321-331. Wang et al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201. Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014. Munger et al. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.