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GluTR | Glutamyl -tRNA reductase

AS10 689  |  Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum

GluTR | Glutamyl -tRNA reductase  in the group Antibodies Plant/Algal  / Photosynthesis  / Chlorophyll at Agrisera AB (Antibodies for research) (AS10 689)
GluTR | Glutamyl -tRNA reductase



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Data sheet Product citations Protocols Add review

Product Information

Immunogen

KLH-conjugated peptide derived from available glutamyl-tRNA reductase sequences including Arabidopsis thaliana P49294

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 200 µl
Reconstitution For reconstitution add 200 µl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

58 | kDa (Arabidopsis thaliana)

Reactivity

Confirmed reactivity Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
Predicted reactivity Brassica napus, Chlamydomonas reinhardtii, Cucumis sativus, Glycine max, Hordeum vulgare, Oryza sativa, Physcomitrium patens, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in Cyanobacteria

Application examples

Application examples

application example

western blot detection using GluTR antibodies

10 ul of leaf extract which was equivalent to 1 mg leaf material was loaded per lane, which may also correspond to approximately 50 ug protein. Arabidopsis thaliana seedlings were grown on vermiculite for 3 weeks under continuous illumination at a light intensity of 80 uE m-2 s-1 at 22°C. Twenty mg leaf material was collected from mature leaves and extracted with 200 ul of the tissue homogenization buffer. 10 ul of leaf extract which was equivalent to 1 mg leaf material was loaded per lane. Detection Protocol: Leaf protein was separated on 14% SDS-PAGE and blotted 2h to PVDF membrane from GE Healthcare. The blot was blocked with PBS-T (PBS plus 0.1% tween 20) containing 3% skim milk for 1h at room temperature (RT: approximately 22 degrees C) with gentle agitation. The blots were briefly washed twice with PBS-T and then incubated with anti-GluTR antibody which was diluted 1:1000 with PBS-T for 1h at RT with agitation. The primary antibody solution was decanted and the blot was rinsed twice, when washed once for 10 min and 3 times for 5 min in PBS-Tcontaining 0.5% (w/v) skim milk at RT with agitation. The blot was incubated with the secondary antibody (HRP-conjugated anti-rabbit IgG) which was diluted 1:20 000 with PBS-T containing 0.5% (w/v) skim milk for 1h at RT with agitation. The blot was washed as described above and incubated with Western Lightning Plus-ECL from Perkin-Elmer for 1 min. The chemiluminescent signal was captured with a CCD camera (LumiVision: Aisin Seiki Inc. Aichi, Japan) for 60 s.

Plant material grown for the experiment illustrated below: barley seeds were sawn on vermiculite and grown for 6 days in darkness at 22°C. Subsequently, seedlings were illuminated for 24 hours under a light intensity of 80 uE m-2 s-1. Approximately, 100 mg of Hordeum vulgare leaf material was harvested before illumination (1), and 2 h (2), 6 h (3) and 24 hours (4) after the onset of light; Arabidopsis thaliana wt (5). Each leaf material was extracted with 1 ml of the tissue homogenization solution (50 mM Tris Cl pH8.0, 2% Lithium Dodesyl Sulfate, 12% sucrose, 1.5% dithiothreitol).

Courtesy of Kaori TAKAHASHI and Ryouichi TANAKA (Hokkaido University)

Additional information

Additional information

Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).

Related products

Background

Background

GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).

Product citations

Selected references Agrawal et al. (2020). The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiol. 2020 Feb 18. pii: pp.00009.2020. doi: 10.1104/pp.20.00009
Montandon et al. (2019). In vivo trapping of proteins interacting with the chloroplast CLPC1 chaperone; potential substrates and adaptors. J Proteome Res. 2019 May 9. doi: 10.1021/acs.jproteome.9b00112.
Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
Picture (footer):

application example

western blot detection using GluTR antibodies

10 ul of leaf extract which was equivalent to 1 mg leaf material was loaded per lane, which may also correspond to approximately 50 ug protein. Arabidopsis thaliana seedlings were grown on vermiculite for 3 weeks under continuous illumination at a light intensity of 80 uE m-2 s-1 at 22°C. Twenty mg leaf material was collected from mature leaves and extracted with 200 ul of the tissue homogenization buffer. 10 ul of leaf extract which was equivalent to 1 mg leaf material was loaded per lane. Detection Protocol: Leaf protein was separated on 14% SDS-PAGE and blotted 2h to PVDF membrane from GE Healthcare. The blot was blocked with PBS-T (PBS plus 0.1% tween 20) containing 3% skim milk for 1h at room temperature (RT: approximately 22 degrees C) with gentle agitation. The blots were briefly washed twice with PBS-T and then incubated with anti-GluTR antibody which was diluted 1:1000 with PBS-T for 1h at RT with agitation. The primary antibody solution was decanted and the blot was rinsed twice, when washed once for 10 min and 3 times for 5 min in PBS-Tcontaining 0.5% (w/v) skim milk at RT with agitation. The blot was incubated with the secondary antibody (HRP-conjugated anti-rabbit IgG) which was diluted 1:20 000 with PBS-T containing 0.5% (w/v) skim milk for 1h at RT with agitation. The blot was washed as described above and incubated with Western Lightning Plus-ECL from Perkin-Elmer for 1 min. The chemiluminescent signal was captured with a CCD camera (LumiVision: Aisin Seiki Inc. Aichi, Japan) for 60 s.

Plant material grown for the experiment illustrated below: barley seeds were sawn on vermiculite and grown for 6 days in darkness at 22°C. Subsequently, seedlings were illuminated for 24 hours under a light intensity of 80 uE m-2 s-1. Approximately, 100 mg of Hordeum vulgare leaf material was harvested before illumination (1), and 2 h (2), 6 h (3) and 24 hours (4) after the onset of light; Arabidopsis thaliana wt (5). Each leaf material was extracted with 1 ml of the tissue homogenization solution (50 mM Tris Cl pH8.0, 2% Lithium Dodesyl Sulfate, 12% sucrose, 1.5% dithiothreitol).

Courtesy of Kaori TAKAHASHI and Ryouichi TANAKA (Hokkaido University)

Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum
predicted reactivity: Brassica napus, Chlamydomonas reinhardtii, Cucumis sativus, Glycine max, Hordeum vulgare, Oryza sativa, Physcomitrium patens, Picea sitchensis, Pisum sativum, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Zea mays, Vitis vinifera
Species of your interest not listed? Contact us
not reactive in: Cyanobacteria
calculated | apparent molecular mass [kDa]:

58 | kDa (Arabidopsis thaliana)

Clonality: Polyclonal
Format: Lyophilized
Host: Rabbit
immunogen:

KLH-conjugated peptide derived from available glutamyl-tRNA reductase sequences including Arabidopsis thaliana P49294

Purity: Serum
Quantity: 200 µl
recommended dilution: 1 : 5000 (WB)
Reconstitution: For reconstitution add 200 µl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
All references: Agrawal et al. (2020). The Functions of Chloroplast Glutamyl-tRNA in Translation and Tetrapyrrole Biosynthesis. Plant Physiol. 2020 Feb 18. pii: pp.00009.2020. doi: 10.1104/pp.20.00009
Montandon et al. (2019). In vivo trapping of proteins interacting with the chloroplast CLPC1 chaperone; potential substrates and adaptors. J Proteome Res. 2019 May 9. doi: 10.1021/acs.jproteome.9b00112.
Nishimura et al. (2013). ClpS1 Is a Conserved Substrate Selector for the Chloroplast Clp Protease System in Arabidopsis. The Plant Cell June 2013.
background:

GluTR (glutamyl tRNA reductase) belongs to a family of oxidoreductases and is involved in porphyrin and chlorophyll biosynthesis. This enzyme class is called L-glutamate-semialdehyde: NADP+ oxidoreductase (L-glutamyl-tRNAGlu-forming).

additional information:

Antibody reacts with recombinant GluTR isoforms: AtGluTR1, AtGluTR2 and NtGluTR1 (At - Arabidopsis thaliana, Nt - Nicotiana tabacum).

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