Glyphosate

AS13 2739 | Clonality: Polyclonal | Host: Chicken | Reactivity: Glyphosate


product information
Background		Glyphosate (N-(phosphonomethyl)glycine) is a herbicide of a broad action spectrum which is used to kill broadleaf weeds and grasses known to compete with commercial crops world-wide. Due to its physico-chemical properties glyphosate easily enters the aqueous environment where it is rapidly converted into its main metabolite AMPA (aminomethylphosphonic acid).
Immunogen		BSA-conjugated Glyphosate (coupled via EDAC/NHS) , Target: Glyphosate, CAS no.: 1071-83-6 from SIGMA.
Host		Chicken
Clonality		Polyclonal
Clone
Purity		IgY
Format		Liquid
Quantity		1 ml (5 mg/ml)
Reconstitution
Storage		Store Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing. For long time storage in 4°C, please add sodium azide 0.02 %.
Tested applications		ELISA (ELISA), Immunoaffinity chromatography (IAC)
Related products		Secondary antibodies
Additional information		Antibody is provided in 0,01M PBS, pH 7,2. Composition of PBS: 8 mM Na2HPO4; 2 mM KH2PO4, 137 mM NaCl; 2,68 mM KCl. For coating OVA-glyphosate or KLH-glyphosate can be used.

application information
Recommended dilution		1 : 2000 (ELISA)
Expected \| apparent MW
Confirmed reactivity
Predicted reactivity
Not reactive in
Additional information		This antibody was used to prepare anti-glyphosate immunoaffinity column which showed to be very specific and retained glyphosate but not AMPA or glufosinate. Recover of glyphosate was ca. 100 %. The antibody is applicable for any type of (waste) water sample. The detection limit is 2,5 μg/l, while the end concentration is 100 μg/l. The standard curve runs from 5 – 100 μg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assay buffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.
Selected references		To be added when available, antibody released in January 2014.

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