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Glyphosate

AS13 2739 | Clonality: Polyclonal | Host: Chicken | Reactivity: Glyphosate
Glyphosate in the group Antibodies for Plant/Algal  / Toxins / Other compounds at Agrisera AB (Antibodies for research) (AS13 2739)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

BSA-conjugated Glyphosate (coupled via EDAC/NHS) , Target: Glyphosate, CAS no.: 1071-83-6 from SIGMA.

Host Chicken
Clonality Polyclonal
Purity IgY
Format Liquid
Quantity 5 mg at 5mg/ml
Storage Store Store at 4°C up to one month or in aliquots at -20°C for long time storage. Avoid repeated freezing and thawing. For long time storage in 4°C, please add sodium azide 0.02 %.
Tested applications ELISA (ELISA), Immunoaffinity chromatography (IAC)
Recommended dilution 1 : 2000 (ELISA)

Reactivity

Application examples

Additional information

Additional information Antibody is provided in 0,01M PBS, pH 7,2. Composition of PBS: 8 mM Na2HPO4; 2 mM KH2PO4, 137 mM NaCl; 2,68 mM KCl.

For coating OVA-glyphosate or KLH-glyphosate can be used.
glyphosate
This antibody was used to prepare anti-glyphosate immunoaffinity column which showed to be very specific and retained glyphosate but not AMPA or glufosinate. Recover of glyphosate was ca. 100 %.

The antibody is applicable for any type of (waste) water sample. The detection limit is 2,5 μg/l, while the end concentration is 100 μg/l. The standard curve runs from 5 – 100 μg/l. For an optimal ELISA result it is crucial to use Greiner high affinity plates; As assay buffer 0,1M PBS, 1% BSA heat-treated, pH 7,4 is recommended.

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Background

Background

Glyphosate (N-(phosphonomethyl)glycine) is a herbicide of a broad action spectrum which is used to kill broadleaf weeds and grasses known to compete with commercial crops world-wide. Due to its physico-chemical properties glyphosate easily enters the aqueous environment where it is rapidly converted into its main metabolite AMPA (aminomethylphosphonic acid).

Product citations

Selected references Viirlaid et al. (2019). Immunoassay for rapid on-site detection of glyphosate herbicide. Environ Monit Assess. 2019 Jul 24;191(8):507. doi: 10.1007/s10661-019-7657-z.

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