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Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated

AS10 774 | Clonality: Polyclonal  |  Host: Goat  | Reactivity: Human IgG (H&L)

Goat anti-Human IgG (H&L), F(ab)'2 fragment, ALP conjugated  in the group Secondary Antibodies / Anti-Human / ALP (alkaline phosphatase) / IgG at Agrisera AB (Antibodies for research) (AS10 774)

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Product Information

Immunogen

Purified human IgG

Host Goat
Clonality Polyclonal
Purity Affinity purified goat IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Reactivity

Confirmed reactivity Human IgG (H&L)
Predicted reactivity Human IgG (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

ALP conjugate is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) of sodium azide is added as preservative.

Purity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

This antibody reacts with the alpha chains on human IgG based on immunoelectrophoresis.

No reactivity is observed to non-immunoglobulin human serum proteins based on immunoelectrophoresis.

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Background

Background

Goat anti-human IgG (H&L), F(ab)'2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L) in immunological assays.

Product citations

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