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Goat anti-Human IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine,mouse,rabbit serum

AS10 1015  | Clonality: Polyclonal  |  Host: Goat  |  Reactivity:Human IgG (H&L)

Goat anti-Human IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine,mouse,rabbit serum in the group Secondary Antibodies / Anti-Human / Fluorescent / IgG at Agrisera AB (Antibodies for research) (AS10 1015)

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Product Information

Host Goat
Clonality Polyclonal
Purity Affinity purified IgG, F(ab)'2 fragment
Format Liquid
Quantity 0.5 mg
Storage Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution 1 : 20-1 : 2000 (IHC),1 : 50-1 : 5000 (ICC),

Reactivity

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Additional information

Additional information

This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.

Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.

Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.

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Background

Background Goat anti-human IgG (H&L) is a FITC  (fluorescein-5-isothiocyanate) conjugated secondary antibody F(ab)'2 fragment, which binds to all human IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed to bovine,mouse,rabbit serum and its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.

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