Goat anti-Human IgG (H&L), F(ab)'2 fragment, FITC conjugated, min. cross-reactivity to bovine,mouse,rabbit serum
AS10 1015 | Clonality: Polyclonal | Host: Goat | Reactivity: Human IgG (H&L)
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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Affinity purified IgG, F(ab)'2 fragment
Format
Liquid
Quantity
0.5 mg
Storage
Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Recommended dilution
1 : 20-1 : 2000 (IHC),1 : 50-1 : 5000 (ICC),
Reactivity
Application examples
Additional information
Additional information
This antibody reacts with the heavy chains on human IgG and with the light chains on all human immunoglobulins based on immunoelectrophoresis.
Minimum cross-reactivity is observed to non-immunoglobulin human, mouse, rabbit or bovine serum proteins based on immunoelectrophoresis.
Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1% (w/v) BSA, protease/IgG free and 0.05 % (w/v) sodium azide as preservative.
Background
Background
Goat anti-human IgG (H&L) is a FITC (fluorescein-5-isothiocyanate) conjugated secondary antibody F(ab)'2 fragment, which binds to all human IgGs in immunological assays. FITC has Amax = 494 nm, Emax = 518 nm. Fluor to protein ratio is 3-7 moles FITC per mole antibody. Antibody is adsorbed to bovine,mouse,rabbit serum and its purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Product citations
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