Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, DyLight® 594 conjugated, min, cross-reactivity to bovine, human, or Mouse lgG or serum proteins

Product no: AS12 2083

AS12 2083 | Clonality: Polyclonal  |  Host: Goat   |   Reactivity: Rabbit IgG (H&L)

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  • Product Info
  • Immunogen:

    Purified rabbit IgG, whole molecule

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 0,5 mg
    Reconstitution: For reconstitution add 0,55 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0,55 ml of sterile water add 0,55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Recommended dilution: 1 : 20-1 : 2000 for most applications
  • Additional Information
  • Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) sodium azide is added as preservative
    Additional information (application):

    Based on  immunoelectrophoresis, this antibody reacts with:  heavy (γ) chains on rabbit IgG  light chains on all rabbit immunoglobulins.

    No reactivity is observed to: non-immunoglobulin rabbit serum proteins IgG from bovine, human or mouse serum proteins from bovine, human or mouse.

  • Background
  • Background:

    Goat anti-rabbit IgG (H&L) -  F(ab)'2 fragment, DyLight® 594 Conjugated, min. cross-reactivity to bovine, human, or mouse lgG or serum proteins is a secondary antibody conjugated to  DyLight® 594, which binds to all rabbit IgG (H&L), F(ab)'2 fragment in immunological assays. 

    DyLight® 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rabbit IgG (H&L).

    DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Antibody protocols
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