Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine/Human/goat/mouse/rat IgG

AS10 667| Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)


product information
Background		Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.
Immunogen		Purified Rabbit IgG, whole molecule (H&L chains)
Host		Goat
Clonality		Polyclonal
Clone
Purity		Affinity purified goat IgG
Format		Lyophilized
Quantity		1 mg
Reconstitution		For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Storage		Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications		Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
Related products		AS09 602 \| Goat anti-rabbit IgG (H&L) HRP conjugated antibodies to rabbit immunoglobulins
Additional information		No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis. No reactivity to non-immunoglobulin rabbit serum proteins. Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase. DyLight™ 488 has a maximum absorbance at 493 nm; Emax = 518 nm Fluor to protein ratio is: 1-5 Moles DyLight™ 488 per Mole Antibody

application information
Recommended dilution		1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)
Expected \| apparent MW
Confirmed reactivity		Rabbit IgG heavy and light chains (H&L)
Predicted reactivity		Rabbit IgG Heavy and Light chains (H&L)
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Selected references		to be added when available

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com