Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG

Product no: AS10 667

AS10 667| Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

206
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Delivery:  7-10 business days
  • Product Info
  • Immunogen:

    Purified Rabbit IgG, whole molecule (H&L chains)

    Host: Goat
    Clonality: Polyclonal
    Purity: Immunogen affinity purified goat IgG.
    Format: Lyophilized
    Quantity: 1 mg
    Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
    Storage: Store lyophilized material at 2-8C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    Tested applications: Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)
  • Reactivity
  • Confirmed reactivity: Rabbit IgG heavy and light chains (H&L)
    Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L)
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information:

    No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.

    No reactivity to non-immunoglobulin rabbit serum proteins.

    Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.

    Additional information (application): No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis,
  • Background
  • Background:

    Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

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