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Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG

AS10 667| Clonality: Polyclonal  |  Host: Goat  | Reactivity: Rabbit IgG (H&L)

10 % until end of 2023. Use discount code: Conj10

Goat anti-Rabbit IgG (H&L), HRP conjugated, min, cross-reactivity to bovine/Human/goat/mouse/rat IgG in the group Secondary Antibodies / Anti-Rabbit / HRP (horse radish peroxidase) at Agrisera AB (Antibodies for research) (AS10 667)



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Product Information

Immunogen

Purified Rabbit IgG, whole molecule (H&L chains)
Host Goat
Clonality Polyclonal
Purity Immunogen affinity purified goat IgG.
Format Lyophilized
Quantity 1 mg
Reconstitution For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
Tested applications Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)

Reactivity

Confirmed reactivity Rabbit IgG heavy and light chains (H&L)
Predicted reactivity Rabbit IgG Heavy and Light chains (H&L)
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information

No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.

No reactivity to non-immunoglobulin rabbit serum proteins.

Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis,

Related products

Background

Background

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

Product citations

immunogen:

Purified Rabbit IgG, whole molecule (H&L chains)
Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Host: Goat
Clonality: Polyclonal
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Quantity: 1 mg
storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
tested applications: Immunohistochemistry (IHC), ELISA (ELISA), Western blot (WB)
recommended dilution: 1 : 200- 1 : 5 000 (ELISA), 1 : 500- 1 : 5 000 (IHC), 1 : 200- 1 : 5 000 (WB)
Confirmed reactivity: Rabbit IgG heavy and light chains (H&L)
predicted reactivity: Rabbit IgG Heavy and Light chains (H&L)
not reactive in: No confirmed exceptions from predicted reactivity are currently known
additional information:

No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.

No reactivity to non-immunoglobulin rabbit serum proteins.

Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
additional information (application): No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis,
background:

Goat anti-rabbit IgG  is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.

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