Goat anti-Rabbit IgG (H&L), HRP conjugated, min. cross-reactivity to bovine/Human/goat/mouse/rat IgG
AS10 667| Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
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Purified Rabbit IgG, whole molecule (H&L chains)
For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity.
For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
No reactivity is observed to bovine/goat/human/mouse or rat IgG based on immunoelectrophoresis.
No reactivity to non-immunoglobulin rabbit serum proteins.
Antibody is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1 % (v/v)Kathon CG is added as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase.
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to all rabbit IgGs in immunological assays.