GPA1 | Guanine nucleotide-binding protein subunit alpha 1

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AS16 3939 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


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Item No:
AS16 3939

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product information
GPA1 (G PROTEIN ALPHA-1) is involved as a modulator or transducer in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade  Promotes abscisic acid (ABA) responses in guard cells. But, together with GCR1 and GB1, acts as a negative regulator of ABA during seed germination and early seedling development. Involved in the blue light (BL) signaling. Alternative name: GP-alpha-1.
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana GPA1, UniProt: P18064, TAIR: AT2G26300
Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AGB1 | Guanine nucleotide-binding protein beta 1
AGB1/AGG1 | Guanine nucleotide-binding protein subunit beta 1 and gamma 1
GPA1 | Guanine nucleotide-binding protein subunit alpha 1
RACK1 | Receptor for activated C kinase 1
RACK1A | Receptor for activated C kinase 1A
other antibodies to signal transduction pathway components

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1: 2000 (WB)
Expected | apparent MW

49.3 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity
Amborella trichopoda, Ananas comosus, Arachis sp., Beta vulgaris,  Brassica sp., Cajanus cajan, Camelina sativa, Capsella rubella, Capsicum annuum, Cicer arietinum, Coffea canephora, Cucumis melo, Daucus carota, Elaeis guineensis, Eucalyptus grandis, Eutrema sp., Fragaria vesca, Genlisea aurea, Glycine max, Glycine soja, Jatropha curcas, Malus domestica, Manihot esculenta, Medicago truncatula, Morus notabilis, Musa acuminata, Nelumbo nucifera, Nicotiana sp., Phoenix dactylifera, Phaseolus vulgaris, Populus sp., Prunus mume, Pyrus sp., Ricinus communis, Sesamum indicum, Solanum sp., Spinacia oleracea, Tarenaya hassleriana, Theobroma cacao, Vigna angularis, Vitis vinifera, Ziziphus jujuba
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

To be added when available, antibody released in July 2016.

 Application example
Western blot using anti-GPA1 antibody
The samples extracted either directly with SDS loading buffer (50mM Tris-HCl pH6.8, 100mM DTT, 2%SDS, 10% glycerol, 0.025% bromophenol blue) and then denatured at 95°C for 5 min, or first extracted with CE buffer (250mM sucrose, 100mM HEPES-KOH pH 7.5, 5% glycerol, 1mM Na2MoO4 x 2H2O, 25mM NaF, 10mM EDTA, 1mM DTT, 0.5%Triton X-100,  protease inhibitor cocktail) and then denatured with SDS loading buffer at 70°C for 5 min. 10 µl of proteins were loaded into each well and separated on 10% SDS-PAGE and blotted 1h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T)  and 5% skimmed milk powder for 1h at room temperature (RT) with agitation. The blot was incubated in the primary antibodies indicated at a dilution of 1: 5 000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 5 times for 15 min in TBS-T with milk powder at RT with agitation. Blot was incubated in secondary antibody (Goat-Anti-Rabbit AP conjugate, Sigma) diluted 1:5000  for 2h at RT with agitation. The blot was washed as above with TBS-T without milk powder, equilibrated in AP buffer (100mM TRIS pH=9.5, 100mM NaCl, 50mM MgCl2) and then developed with BioRad Immunstar AP substrate, before exposure to a CEA RP NEW film for 20 s.

Courtesy of Dr. Elena Petusching, Georg-August-University Goettingen, Germany

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