GAL | Beta galactosidase
AS20 4515 | Clonality: Monoclonal | Host: Mouse | Reactivity: Beta Galactosidase (E.coli)
|Data sheet||Product citations||Protocols||Add review|
1. Purified β-galactosidase, 10 ng
2. Uninduced E. coli K12 cell extract (30 ug)
3. E.coli K12 cell extract induced by IPTG for β-galactosidase expression (30 ug)
The anti-β-galactosidase antibody was used at 1:1000 dilution in TBS-T.
Molecular mass of β-galactosidase is 116 kDa.
Sample: Crude extract of E. coli K12 cells induced by IPTG for β-galactosidase expression.
1. Crude cell extract
2. Supernatant of the immuno-precipitated E. coli crude extract
3. Immuno-precipitate of crude E. coli extract
The anti-β-galactosidase antibody was used at 1:500 dilution for immune-precipitation and 1:1000 for western blot. IgG (H) is heavy chain of IgG immunoglobulin. Membrane for transfer: nitrocellulose. Detection: chemiluminescent, following manufacture's recommendations.
HEK293A cells were transfected with β -Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with anti-β-Gal antibody (1: 500) and Alexa 555-conjugated rabbit IgG (1:500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody reacts only in transfected cells.
Related products: GAL | Beta galactosidase
This product can b...