H3 | Histone H3 (affinity purified)

PRODUCT INFORMATION IN PDF

product information Background Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4. Immunogen KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890) Host Rabbit Clonality Polyclonal Clone Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized in PBS pH 7.4 Quantity 50 µg Reconstitution For reconstitution add 50 µl of sterile water. Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications ChIp-qPCR (ChIp-qPCR), Western blot (WB) Related products AS11 1804 | anti-RNA polymerase II subunit B1, rabbit antibody AS16 3197 | anti-H3 | Histone 3 core, monoclonal antibody AS15 2855 | anti-H3 | histone H3, chicken antibody AS10 710 | anti-H3 | histone H3, rabbit antibody (serum) AS10 710PRE | H3 | histone H3, pre-immune serum Collection of antibodies to epigenetics/DNA methylation Plant and algal protein extraction buffer Secondary antibodies Additional information Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

application information
Recommended dilution		2.5 µg/100 µg of chromatin (ChIp-qPCR), 1 : 5000 (WB)
Expected | apparent MW		15 | 17 kDa
Confirmed reactivity		Arabidopsis thaliana
Predicted reactivity		Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.
Not reactive in		No confirmed exceptions from predicted reactivity are currently known.
Additional information		Protocol for isolation of cytosolic and nuclear fractions can be found here. Specific protol for ChIP can be found here: Saleh et al. (2008).
Selected references		To be added when available, antibody released in April 2016.

Application example




Chromatin Immunoprecipitation: using anti-plant Histone 3 polyclonal antibodies. Chromatin from Arabidopsis thaliana wilde type, deacetylase mutant and over-expressors was cross-linked using formaldehyde. Chromatin was isolated and DNA was sheared along with the bound protein by sonictaion. DNA-protein complex was immunoprecipitated using affinity purified, polyclonal anti-Histone 3 antibodies. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Procedure was according to a protocol described here: Saleh et al. (2008).

Courtesy of Dr. Cristián Holzmann, Catholic University of Chile, Chile

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