H3 | Histone H3 (affinity purified)

AS10 710A | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, S. lycopersicum, V. faba, P. patens, S. europaea, Z. mays, Ch. reinhardtii | cellular [compartment marker] of nucleoplasm

Product Information

Immunogen

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host Rabbit

Clonality Polyclonal

Purity Affinity purified serum in PBS, pH 7.4

Format Lyophilized

Quantity 50 µg

Reconstitution For reconstitution add 50 µl of sterile water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ChIp-qPCR (ChIp-qPCR), Western blot (WB)

Recommended dilution 2.5 µg/100 µg of chromatin (ChIp-qPCR), 1 : 5000 (WB)

Expected | apparent MW

15 | 17 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana

Predicted reactivity

Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays Brachypodium distachyon, Brassica napus, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Nannochloropsis gaditana, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.

Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples Application example

IP using polyclonal anti-plant H3 antibody

Chromatin Immunoprecipitation: using anti-plant Histone 3 polyclonal antibodies. Chromatin from Arabidopsis thaliana wilde type, deacetylase mutant and over-expressors was cross-linked using formaldehyde. Chromatin was isolated and DNA was sheared along with the bound protein by sonictaion. DNA-protein complex was immunoprecipitated using affinity purified, polyclonal anti-Histone 3 antibodies. Immunoprecipitated DNA was quantified using quantitative PCR and normalized to the input chromatin.
Procedure was according to a protocol described here: Saleh et al. (2008).

Courtesy of Dr. Cristián Holzmann, Catholic University of Chile, Chile

Additional information

Additional information Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

Protocol for isolation of cytosolic and nuclear fractions can be found here.

Specific protol for ChIP can be found here: Saleh et al. (2008).

Related products

AS10 710PRE | H3 | histone H3, pre-immune serum (control for immunolocalization)

Collection of antibodies to epigenetics/DNA methylation

Plant and algal protein extraction buffer

Background

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

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