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H3K18me3 | Histone H3 (trimethylated Lys18)

AS16 3183 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
H3K18me3 | Histone H3 (trimethylated Lys18) in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Epigenetics/DNA methylation at Agrisera AB (Antibodies for research) (AS16 3183)

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Product Information

Immunogen KLH-conjugated synthetic peptide
Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum
Format Liquid
Quantity 50 µg
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
Recommended dilution 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
Expected | apparent MW

15 kDa

Reactivity

Confirmed reactivity Human
Predicted reactivity Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Application examples application example

dot blot using anti-H3K18me3 | Histone H3 (trimethylated Lys18) antibodies

Dot Blot: using anti-H3K18me3 antibody. Lane 1: K18 unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 0.5 μg/ml for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

immunofluorescence using anti-H3K18me3 | Histone H3 (trimethylated Lys18) antibodies


Immunofluorescence: using anti-H3K18me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K18me3 is nuclear and chromosomal. Staining: Histone H3K18me3 is expressed in green, nuclei are counterstained with DAPI (blue).


western blot using anti- H3K18me3 | Histone H3 (trimethylated Lys18) antibodies




Western Blot: using anti-H3K18me3 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. Predicted/Observed size: ~15 kDa.

Additional information

Additional information This antibody preparation is provided in 20 mM Potassium Phosphate pH 7.2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol.

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collection of antibodies to epigenetics

Background

Background Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.

Product citations

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