IKP

Anti-H3K18me3 | Histone H3 (trimethylated Lys18)

Product no: AS16 3183
AS16 3183 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549
Add to cart
Delivery:  3-6 business days
  • Product Info
  • Immunogen: KLH-conjugated synthetic peptide
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum.
    Format: Liquid
    Quantity: 50 g
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)
    Recommended dilution: 2-5 µg/million cells (ChIP), 1 : 1000 (Dot), 1 : 50 (IF), 1 : 50 (IHC), 1 : 500 (WB)
    Expected | apparent MW:

    15 kDa

  • Reactivity
  • Confirmed reactivity: Human
    Predicted reactivity: Caenorhabditis elegans, Chicken, Drosophila melanogaster, Mouse, Plant, Rat, Xenopus sp.
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    dot blot using anti-H3K18me3 | Histone H3 (trimethylated Lys18) antibodies

    Dot Blot: using anti-H3K18me3 antibody. Lane 1: K18 unmodified. Lane 2: K18Me1. Lane 3: K18Me2. Lane 4: K18Me3. Lane 5: K18Ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody used at 0.5 μg/ml for 45 min at 4°C. Secondary antibody: Dylight®488 rabbit secondary antibody at 1:10 000 for 45 min at RT.

    immunofluorescence using anti-H3K18me3 | Histone H3 (trimethylated Lys18) antibodies


    Immunofluorescence: using anti-H3K18me3 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Primary antibody used at a 1:50 dilution for 1 h at RT. Secondary antibody: FITC secondary antibody at 1:10 000 for 45 min at RT. Localization: Histone H3K18me3 is nuclear and chromosomal. Staining: Histone H3K18me3 is expressed in green, nuclei are counterstained with DAPI (blue).


    western blot using anti- H3K18me3 | Histone H3 (trimethylated Lys18) antibodies




    Western Blot: using anti-H3K18me3 antibody. 30 μg of C. elegans embryo lysate. Primary antibody used at a 1:500 dilution overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10 000 for 45 min at RT. Predicted/Observed size: ~15 kDa.
  • Additional Information
  • Additional information: This antibody preparation is provided in 20 mM Potassium Phosphate pH 7,2, 150 mM NaCl, 0,01% sodium azide and 30% glycerol
  • Background
  • Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
  • Reviews:
  • Reviews
    Below, you can grade the product on a scale from 0 to 5.
    Please also provide information about species, application, dilution and obtained result for the reviewed antibody.
    Your name will be displayed as the sender.
    Number of reviews: (0)

    This product doesn't have any reviews.

Accessories

AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

213 €
Add to cart
Info
AS16 3182 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
549 €
Add to cart
Info
AS16 3180 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Chicken, C.elegans, D. melanogaster, Human, Mouse, plant, Rat, Xenopus sp.
523 €
Add to cart
Info
Close