H+ATPase | Plasma membrane H+ATPase (rabbit antibody), ALP-conjugated (40 µg)

Product no: AS07 260-ALP

AS07 260-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for di- and monocots, conifers, ferns, mosses, green algae | Cellular [compartment marker] for plasma membrane

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide, derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (UniProt: P20649, TAIR: At2g18960) and ATPase 2 (UniProt: P19456 , TAIR: At4g30190), 3 (UniProt: P20431, TAIR: At5g57350), 4 (UniProt: Q9SU58, TAIR: At3g47950), 6 (UniProt: Q9SH76, TAIR: At2g07560), 7 (UniProt: Q9LY32, TAIR: At3g60330), 8 (UniProt: Q9M2A0, TAIR: At3g42640), 9 (UniProt: Q42556, TAIR: At1g80660), 11 (UniProt: Q9LV11, TAIR: At5g62670) of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Antigen affinity purified serum in PBS pH 7.4, conjugated to ALP.
    Format: Liquid
    Quantity: 40 µg
    Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.
    Tested applications: Immunolocalization (IL), Western blot (WB)
    Recommended dilution: 1 : 100 (IL), 1 : 1000-1 : 5000 (WB)
    Expected | apparent MW: 90- 95 kDa (Arabidopsis thaliana, depending upon an isoform)
  • Reactivity
  • Confirmed reactivity: Actinidia chinensis, Aesculus hippocastanum, Arabidopsis thaliana, Camellia sinensis cv. Shu-chazao, Chara australis R.Br, Chlamydmonas reinhardtii, Cucumis sativus, Cucurbita moschata, Glycine max, Kandelia obovata, Hordeum vulgare, Lolium perenne, Lycopersicon esculentum, Malus x domestica Borkh. c.v. Fuji, Marchantia polymorpha, Medicago truncatula, Nicotiana benthamiana, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida, Phalenopsis Sogo Yukidian cultivar V3, Physcomitrium patens, Picea abies, Pisum sativum, Populus tremula, Pteris vittata (fern), Ricinus communis, Spinacia oleracea, Solanum lycopersicum, Tagetes erecta, Tetraselmis chuii, Zea mays, Vicia faba
    Predicted reactivity: Algae, Amaranthus hypochondriacus,Avena sativa,Beta vulgaris, Cyanidioschyzon merolae, Dunaliella spp., Gossypium hirsutum, Hordeum vulgare, Ostreococcus spp., Pinus thunbergii, Physocomitrella patens, Mesembruanthemum crystallinum, Mortierella elongata, Nannochloropsis gaditana CCMP526, Ostreococcus tauri, Prosopis alba, Saccharomyces cerevisiae, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Ulva prolifera, Ustilago maydis

    Species of your interest not listed? Contact us
    Not reactive in:

    Allium sp., Aspergillus niger, Citrus limon, Colobanthus apetala, Cuminium cyminum, Curcuma amada, Deschampsia antractica, Lupinus luteus, Morinda citrifolia, Trigonella foenum, Vicia faba

  • Additional Information
  • Additional information:

    Cellular [compartment marker] for plasma membrane

    Additional information (application):
    VERY IMPORTANT: Please, do not heat up your samples above 70°C as this may cause H+ATPase to precipitate, and there will be no signal on your Western Blot.

    Before SDS-PAGE, centrifuge your samples at room temperature at 10 000 rpm/1 min to remove any aggregates. 

    H+ATPase will be less abundant in mature roots and leafs, and therefore detection may require use of very sensitive reagents. 

     

  • Background
  • Background:

    The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

    This antibody is directly conjugated to ALP.

  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Plasma membrane protein isolation protocol
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Accessories

AS07 213  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Higher plants including A.comosus, A.thaliana, C.sativus, C. australis R.Br, C.reinhardtii, F. margarita Swingle, H.vulgare, L.esculentum, L.longiflorum, Malus x domestica Borkh. c.v. Fuji, M. truncatula, M.crystallinum, N.tabacum, N.caerulescens, O.sativa, P.hybrida cv. Mitchell, Populus sp., P.vittata, Thellungiella sp., T. aestivum, Z.mays, V. vinifera |  Cellular [compartment marker] of tonoplast membrane

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Buy 2 items of this product for 226.00 €/items
Buy 3 items of this product for 205.00 €/items
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