Histone H2B (Schizosaccharomyces pombe)

Name: Histone H2B (Schizosaccharomyces pombe)
SKU: AS21 4558
Price: 311 EUR
Availability: InStock

AS21 4558 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Schizosaccharomyces pombe

Histone H2B (Schizosaccharomyces pombe) in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS21 4558)

Product Information

Immunogen Synthetic peptide corresponding to N-terminal Schizosaccharomyces pombe histone H2B, SAAEKKPASKAPAGKA, UniProt: P04913

Host Rabbit

Clonality

Polyclonal

Purity Serum. Contains 0.05 % sodium azide.

Format Liquid

Quantity 50 µl

Storage Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Chromatin immunoprecipitation (ChIP), Western blot (WB)

Recommended dilution 1 : 1000 (WB)

Expected | apparent MW 13,8 | 17, 24-25 kDa (unmodified and mono-ubiquinated H2B)

Reactivity

Confirmed reactivity Schizosaccharomyces pombe

Predicted reactivity Species of your interest not listed? Contact us

Application examples

Application examples Western blot using anti-histone H2B (yeast) antibodies

Crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations. Described in Maruyama et al. (2006).

Additional information

ChIP method for this antibody is described in Maruyama et al. (2006).

Background

Background In the eukaryotic cells, DNA is packaged repetitively into nucleosomes by means of interactions among two molecules of four classes of histone, H2A, H2B, H3 and H4. Each of the histone proteins has an evolutionarily conserved amino-terminal ‘tail’ that protrudes from the nucleosome. This tail is the target of numerous diverse signaling pathways, resulting in the addition of many post-translational modifications. These modifications include phosphorylation, acetylation, methylation, ADP-ribosylation and mono-ubiquitination. Many important new modifications within the structured core and the carboxy-terminal tail regions of histones are also being identified. It is becoming increasingly clear that these modifications represent crucial regulatory events that govern the accessibility and function of the genome.

Product citations

Selected references Maruyama et al (2006). Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation. EMBO J. 2006 Jun 7;25(11):2420-31. doi: 10.1038/sj.emboj.7601110. Epub 2006 May 11. PMID: 16688222; PMCID: PMC1478186.

immunogen:	Synthetic peptide corresponding to N-terminal Schizosaccharomyces pombe histone H2B, SAAEKKPASKAPAGKA, UniProt: P04913
Host:	Rabbit
Clonality:	Polyclonal
Purity:	Serum. Contains 0.05 % sodium azide.
Format:	Liquid
Quantity:	50 µl
storage:	Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Chromatin immunoprecipitation (ChIP), Western blot (WB)
recommended dilution:	1 : 1000 (WB)
calculated \| apparent molecular mass [kDa]:	13,8 \| 17, 24-25 kDa (unmodified and mono-ubiquinated H2B)
Confirmed reactivity:	Schizosaccharomyces pombe
predicted reactivity:	Species of your interest not listed? Contact us
Picture (footer):	Crude extract of Schizosaccharomyces pombe was separated on SDS-PAGE and blotted to a membrane using wet transfer. Primary antibody was incubated at 1: 1000, followed by washes and incubation with a secondary goat anti-rabbit IgG HRP conjugated antibodies, used at 1: 10 000 1h/RT. Reaction was developed using chemiluminescence following manufacture's recommendations. Described in Maruyama et al. (2006).
additional information (application):	ChIP method for this antibody is described in Maruyama et al. (2006).
background:	In the eukaryotic cells, DNA is packaged repetitively into nucleosomes by means of interactions among two molecules of four classes of histone, H2A, H2B, H3 and H4. Each of the histone proteins has an evolutionarily conserved amino-terminal ‘tail’ that protrudes from the nucleosome. This tail is the target of numerous diverse signaling pathways, resulting in the addition of many post-translational modifications. These modifications include phosphorylation, acetylation, methylation, ADP-ribosylation and mono-ubiquitination. Many important new modifications within the structured core and the carboxy-terminal tail regions of histones are also being identified. It is becoming increasingly clear that these modifications represent crucial regulatory events that govern the accessibility and function of the genome.
All references:	Maruyama et al (2006). Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation. EMBO J. 2006 Jun 7;25(11):2420-31. doi: 10.1038/sj.emboj.7601110. Epub 2006 May 11. PMID: 16688222; PMCID: PMC1478186.