Anti-HRP | Horseradish peroxidase (mouse monoclonal) (clone HP-03)

Product no: AS21 4617
AS21 4617 | Clonality: Monoclonal | Host: Mouse | Reactivity: Armoracia rusticana and HRP-tagged proteins
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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Horseradish peroxidase (Grade I)
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: Purified by precipitation and chromatography.
    Format: Liquid at 1 mg/ml.
    Quantity: 0.1 mg
    Storage: Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Immunocyto chemistry (ICC), Western blot (WB)
    Recommended dilution: 1 µg/ml (WB)
  • Reactivity
  • Confirmed reactivity: Armoracia rusticana, HRP-tagged, recombinant proteins
  • Application Examples
  • Western blot using anti-HRP monoclonal antibodies
    Purified horse radish peroxidase was separated on SDS gel and transferred to a membrane. Sample was prepared in non-reducing conditions. Primary antibodies were used in dilution 1 µg/ml, followed by washes and incubation with a secondary anti-mouse IgG antibody. Reaction was visualized with chemiluminescence, following manufacture's recommendations.
  • Additional Information
  • Additional information: The antibody binds horseradish peroxidase, It is suitable for preparation of PAP (Peroxidase-Anti-Peroxidase soluble complexes), where three molecules of HRP are complexed with two molecules of anti-HRP antibodies
    Additional information (application): Immunocytochemistry: this was successfully used for staining of formaldehyde-fixed, Triton-permeabilized cells transfected with HRP gene
  • Background
  • Background: Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress. HRP is also used as an epitope tag, for protein overexpression. 
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Antibody Protocols
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AS09 627 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Mouse IgG (H&L)

218 €
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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

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From 17 €