HRP | Horseradish peroxidase (mouse monoclonal) (clone HP-03)
AS21 4617 | Clonality: Monoclonal | Host: Mouse | Reactivity: Armoracia rusticana and HRP-tagged proteins

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Product Information
Immunogen
Horseradish peroxidase (Grade I)
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
Purified by precipitation and chromatography.
Format
Liquid at 1 mg/ml.
Quantity
0,1 mg
Storage
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
ELISA (ELISA), Immunocyto chemistry (ICC), Western blot (WB)
Recommended dilution
1 µg/ml (WB)
Reactivity
Confirmed reactivity
Armoracia rusticana, HRP-tagged, recombinant proteins
Application examples
Application examples

Purified horse radish peroxidase was separated on SDS gel and transferred to a membrane. Sample was prepared in non-reducing conditions. Primary antibodies were used in dilution 1 µg/ml, followed by washes and incubation with a secondary anti-mouse IgG antibody. Reaction was visualized with chemiluminescence, following manufacture's recommendations.

Purified horse radish peroxidase was separated on SDS gel and transferred to a membrane. Sample was prepared in non-reducing conditions. Primary antibodies were used in dilution 1 µg/ml, followed by washes and incubation with a secondary anti-mouse IgG antibody. Reaction was visualized with chemiluminescence, following manufacture's recommendations.
Additional information
Additional information
The antibody binds horseradish peroxidase, It is suitable for preparation of PAP (Peroxidase-Anti-Peroxidase soluble complexes), where three molecules of HRP are complexed with two molecules of anti-HRP antibodies
Immunocytochemistry: this was successfully used for staining of formaldehyde-fixed, Triton-permeabilized cells transfected with HRP gene
Background
Background
Horseradish peroxidase removes hydrogen peroxide, acts in oxidation of toxic reductants, biosynthesis and degradation of lignin, response to environmental stresses such as wounding, pathogen attack and oxidative stress. HRP is also used as an epitope tag, for protein overexpression.
Product citations
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