WHIRLY1
AS16 3953 | Clonality: Polyclonal | Host: Goat | Reactivity: Hordeum vulgare
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Product Information
Host
Goat
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4.
Format
Lyophilized
Quantity
50 ĩg
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunogold (IG)
Recommended dilution
1: 200 (IG)
Reactivity
Confirmed reactivity
Hordeum vulgare
Predicted reactivity
Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartu
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Application example
Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White
Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-W1 (goat) 1:200 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, rabbit anti-goat (10 nm gold) von Aurion (Code 810077)
5. Washing: 3x 5 min PBS
6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins
Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany
Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White
Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-W1 (goat) 1:200 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, rabbit anti-goat (10 nm gold) von Aurion (Code 810077)
5. Washing: 3x 5 min PBS
6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins
Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany
Additional information
Background
Background
WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
Product citations
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AS16 3952 | Clonality: Polyclonal | Host: Chicken | Reactivity: Hordeum vulgare
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