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WHIRLY1
Product no: AS16 3953
AS16 3953 | Clonality: Polyclonal | Host: Goat | Reactivity: Hordeum vulgare
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- Product Info
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Immunogen: KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified serum in PBS pH 7.4. Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution add 50 µl, of sterile water Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunogold (IG) Recommended dilution: 1: 200 (IG) - Reactivity
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Confirmed reactivity: Hordeum vulgare Predicted reactivity: Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartu
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White
Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-W1 (goat) 1:200 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, rabbit anti-goat (10 nm gold) von Aurion (Code 810077)
5. Washing: 3x 5 min PBS
6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins
Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany - Background
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Background: WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673). - Product Citations
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Selected references: Karpinska et al. (2022). WHIRLY1 functions in the nucleus to regulate barley leaf development and associated metabolite profiles. Biochem J. 2022 Mar 18;479(5):641-659. doi: 10.1042/BCJ20210810. PMID: 35212355; PMCID: PMC9022988.
- Protocols
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Protocols to work with plant and algal protein extracts
Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela
Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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