AS16 3953   | Clonality: Polyclonal |  Host: Goat | Reactivity: Hordeum vulgare

WHIRLY1 in the group Antibodies Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS16 3953)


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Product Information

Immunogen KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI
Host Goat
Clonality Polyclonal
Purity Immunogen affinity purified serum in PBS pH 7.4.
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunogold (IG)
Recommended dilution 1: 200 (IG)


Confirmed reactivity Hordeum vulgare
Predicted reactivity Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartu
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Immunogold labelling using anti-HvWHIRLY2 antibodies

Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White  
Samples: Ultrathin sections on Au-Grids  
Immunogold labelling:  
1. Blocking   30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated  
2. Incubation over night at 4°C anti-W1 (goat) 1:200 in blocking buffer  
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)  
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, rabbit anti-goat (10 nm gold) von Aurion (Code 810077) 
5. Washing: 3x 5 min PBS  
6. Washing 4x2 min mit A. millipore,  staining in saturated aqueous uranyl acetate 10 mins

Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany

Additional information

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Background WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).

Product citations

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AS16 3952   | Clonality: Polyclonal |  Host:  Chicken | Reactivity: Hordeum vulgare
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Buy 2 items of this product for 262.00 €/items
Buy 3 items of this product for 205.00 €/items