Name:
Phone:
E-mail:
Message


 


WHIRLY1

AS16 3953   | Clonality: Polyclonal |  Host: Goat | Reactivity: Hordeum vulgare

WHIRLY1 in the group Antibodies for Plant/Algal  / DNA/RNA/Cell Cycle / Transcription regulation at Agrisera AB (Antibodies for research) (AS16 3953)

DATA SHEET IN PDF

Qty: 
286
Buy 2 items of this product for 249.00 €/items
Buy 3 items of this product for 229.00 €/items
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Protocols Add review

Product Information

Immunogen KLH-conjugated peptide derived from HvWHIRLY1 protein sequence, NCBI
Host Goat
Clonality Polyclonal
Purity Affinity purified serum, in PBS pH 7.4
Format

Lyophilized

Quantity

50 µg

Reconstitution

For reconstitution add 50 µl, of sterile water.

Storage

Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks.
Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunogold (IG)
Recommended dilution 1: 200 (IG)

Reactivity

Confirmed reactivity Hordeum vulgare
Predicted reactivity Brachypodium sylvaticum, Oryza sativa, Panicum miliaceum, Setaria italica, Triticum urartu
Species of your interest not listed? Contact us
Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example


Immunogold labelling using anti-HvWHIRLY2 antibodies


Material: Barley(flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White  
Samples: Ultrathin sections on Au-Grids  
Immunogold labelling:  
1. Blocking   30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated  
2. Incubation over night at 4°C anti-W1 (goat) 1:200 in blocking buffer  
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)  
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, rabbit anti-goat (10 nm gold) von Aurion (Code 810077) 
5. Washing: 3x 5 min PBS  
6. Washing 4x2 min mit A. millipore,  staining in saturated aqueous uranyl acetate 10 mins

Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany

Additional information

Related products

Related products AS16 3952 | Anti-WHIRLY2, chicken antibodies (suitable for western blot)

Background

Background WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).

Product citations

Related products: WHIRLY1

AS16 3952   | Clonality: Polyclonal |  Host:  Chicken | Reactivity: Hordeum vulgare
286 €
Buy 2 items of this product for 249.00 €/items
Buy 3 items of this product for 229.00 €/items