WHIRLY2

Product no: AS16 3952

AS16 3952   | Clonality: Polyclonal |  Host:  Chicken | Reactivity: Hordeum vulgare

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI
    Host: Chicken
    Clonality: Polyclonal
    Purity: Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at 4C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunogold (IG), Western blot (WB)
    Recommended dilution:

    1: 100 (IG), 1: 1500 (WB)

    Expected | apparent MW:

     25 kDa

  • Reactivity
  • Confirmed reactivity: Hordeum vulgare
    Predicted reactivity: Brachypodium distachyon, Triticum urartu
    Species of your interest not listed? Contact us
    Not reactive in:

    Glycine max

  • Application Examples
  • Application example



    Immunogold labeling using chicken anti-WHIRLY2 antibodies

    Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: Ultrathin sections on Au-Grids
    Immunogold labelling:
    1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
    2. Incubation over night at 4°C anti-WHIRLY2 (chicken antibodies) 1:100 in blocking buffer
    3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
    4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, donkey anti-chicken (18 nm gold) 
    5. Washing: 3x 5 min PBS 6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins

    Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany
  • Background
  • Background: WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts


    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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