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Product Information
Host
Chicken
Clonality
Polyclonal
Purity
Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.
Format
Liquid
Quantity
50 ĩg
Storage
Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Immunogold (IG), Western blot (WB)
Recommended dilution
1: 100 (IG), 1: 1500 (WB)
Expected | apparent MW
25 kDa
Reactivity
Confirmed reactivity
Hordeum vulgare
Predicted reactivity
Brachypodium distachyon, Triticum urartu
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
Glycine max
Application examples
Application examples
Application example

Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-WHIRLY2 (chicken antibodies) 1:100 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, donkey anti-chicken (18 nm gold)
5. Washing: 3x 5 min PBS 6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins
Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany

Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-WHIRLY2 (chicken antibodies) 1:100 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, donkey anti-chicken (18 nm gold)
5. Washing: 3x 5 min PBS 6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins
Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany
Additional information
Background
Background
WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
Product citations
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