WHIRLY2

AS16 3952 | Clonality: Polyclonal | Host: Chicken | Reactivity: Hordeum vulgare

Product Information

Immunogen KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI

Host Chicken

Clonality Polyclonal

Purity Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.

Format Liquid

Quantity 50 µg

Storage Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunogold (IG), Western blot (WB)

Recommended dilution

1: 100 (IG), 1: 1500 (WB)

Expected | apparent MW

25 kDa

Reactivity

Confirmed reactivity Hordeum vulgare

Predicted reactivity Brachypodium distachyon, Triticum urartu
Species of your interest not listed? Contact us

Not reactive in

Glycine max

Application examples

Application examples Application example

Immunogold labeling using chicken anti-WHIRLY2 antibodies

Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: Ultrathin sections on Au-Grids
Immunogold labelling:
1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated
2. Incubation over night at 4°C anti-WHIRLY2 (chicken antibodies) 1:100 in blocking buffer
3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer)
4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, donkey anti-chicken (18 nm gold)
5. Washing: 3x 5 min PBS 6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins

Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany

Additional information

Background

Background WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).

Product citations

Picture (footer):	Application example Material: Hordeum vulgare (flag leaves), fixed in 4% formaldehyde (from paraformaldehyde), embedded in LR White Samples: Ultrathin sections on Au-Grids Immunogold labelling: 1. Blocking 30 min RT in PBS (pH 7,4) + 0,2% BSA + 0,2 % fish gelatin (= blocking buffer) sterile filtrated 2. Incubation over night at 4°C anti-WHIRLY2 (chicken antibodies) 1:100 in blocking buffer 3. Washing: 4x 3 min PBS + 0,1 % BSA (= washing buffer) 4. Incubation 60 min RT, dilution: 1:50 in blocking buffer, donkey anti-chicken (18 nm gold) 5. Washing: 3x 5 min PBS 6. Washing 4x2 min mit A. millipore, staining in saturated aqueous uranyl acetate 10 mins Courtesy of Dr. Karin Krupinska, Christian-Albrechts-Universität, Kiel, Germany
calculated \| apparent molecular mass [kDa]:	25 kDa
Clonality:	Polyclonal
Format:	Liquid
Host:	Chicken
immunogen:	KLH-conjugated peptide derived from HvWHIRLY2 protein sequence, NCBI
Purity:	Immunogen affinity purified IgY in PBS pH 8 with 0.02 % sodium azide.
Quantity:	50 µg
recommended dilution:	1: 100 (IG), 1: 1500 (WB)
storage:	Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Immunogold (IG), Western blot (WB)
background:	WHIRLY proteins are multifunctional DNA/RNA-binding proteins (Prikryl et al. 2008, NAR 36: 5152-5165). WHIRLY1 was shown to be located both in chloroplasts and nucleus (Grabowski et al. 2008, Plant Physiology 147:1800-1804). In chloroplasts it is part of nucleoids (Pfalz et al. 2006, Plant Cell 18:176-197; Melonek et al. 2010, Planta 232:471-481). In the nucleus, WHIRLY1 is an acitivator of pathogen response genes (Desveaux et al. 2000, Plant Cell 12:1477-1489). WHIRLY2 is a mitochondrial protein and affects DNA copy number (Cai et al. 2015, Plant Physiology 169:660-673).
Confirmed reactivity:	Hordeum vulgare
predicted reactivity:	Brachypodium distachyon, Triticum urartu Species of your interest not listed? Contact us
not reactive in:	Glycine max