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HXK1 | Hexokinase 1

AS12 2601 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum

HXK1 | Hexokinase 1 in the group Antibodies for Plant/Algal  / Developmental Biology / Signal transduction at Agrisera AB (Antibodies for research) (AS12 2601)

DATA SHEET IN PDF

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How to cite this product:
Product name, number (Agrisera, Sweden)

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana hexokinase-1, UniProt: Q42525, TAIR:AT4G29130

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

53 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Pisum sativum
Predicted reactivity Actinidia chinensis, Arabis alpina, Brassica napus, Camellia sinensis, Capsella rubella, Coffea canephora, Cucumis sativus, Dimocarpus longan, Eucalyptus grandis, Glycine max, Gossypium raimondii, Jatropha curcas, Malus domestica, Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Species of your interest not listed? Contact us
Not reactive in

Chlamydomonas reinhardtii, Saccharomyces cerevisiae

Application examples

Application examples

application example

western blot using anti-hexokinase antibodies

ca. 4 µg and 10 µg of total protein (outer envelop of chloroplasts) from Pisum sativum leaves extracted with 20m M Mops, 13 mM Tris, 0.1 mM MgCl2 330 mM Sorbit 0.02% BSA (stored in NaPO4) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 1% milk 1x TBS-T for 3x10 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody(goat anti-rabbit IgG, HRP conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk1xTBS-T for 1h at RT with agitation. The blot was washed as above and developed for 1 min with combination of 100 mM Tris-HCL pH 8.5, 1%Luminol, 0.44% Coomaric Acid and 100 mM Tris-HCl pH 8.5, 0.018% H2O2 (1mL of each Solution, selfmade). Exposure time was 60 seconds.

Courtesy of Bettina Mathes, Ludwig Maximilians University Munich, Germany

Additional information

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Background

Background

HXK1 (Hexokinase 1) is an enzyme of plant glucose-signaling network which functions as a glucose sensor.

Alternative name: Protein GLUCOSE INSENSITIVE 2.

Product citations

Selected references Gil et al. (2017) ZEITLUPE Contributes to a Thermoresponsive Protein Quality Control System in Arabidopsis. PlantCell. 2017 Nov;29(11):2882-2894. doi: 10.1105/tpc.17.00612.

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