HXK1 | Hexokinase 1

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AS12 2601 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum


25 st
Item No:
AS12 2601

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product information

HXK1 (Hexokinase 1) is an enzyme of plant glucose-signaling network which functions as a glucose sensor. Alternative name: Protein GLUCOSE INSENSITIVE 2.


KLH-conjugated synthetic peptide derived from Arabidopsis thaliana hexokinase-1, UniProt: Q42525, TAIR:AT4G29130

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

collection of antibodies to carbohydrate metabolism

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 1000 (WB)
Expected | apparent MW

53 kDa

Confirmed reactivity Arabidopsis thaliana, Pisum sativum
Predicted reactivity Actinidia chinensis, Arabis alpina, Brassica napus, Camellia sinensis, Capsella rubella, Coffea canephora, Cucumis sativus, Dimocarpus longan, Eucalyptus grandis, Glycine max, Gossypium raimondii, Jatropha curcas, Malus domestica, Nicotiana benthamiana, Nicotiana tabacum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Not reactive in

Chlamydomonas reinhardtii, Saccharomyces cerevisiae

Additional information
Selected references

to be added when available, antibody released in December 2015

application example

western blot using anti-hexokinase antibodies

ca. 4 µg and 10 µg of total protein (outer envelop of chloroplasts) from Pisum sativum leaves extracted with 20m M Mops, 13 mM Tris, 0.1 mM MgCl2 330 mM Sorbit 0.02% BSA (stored in NaPO4) were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry. Blots were blocked with 1% milk 1x TBS-T for 3x10 min at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody(goat anti-rabbit IgG, HRP conjugated, from Agrisera, AS09 602) diluted to 1:25 000 in 1% milk1xTBS-T for 1h at RT with agitation. The blot was washed as above and developed for 1 min with combination of 100 mM Tris-HCL pH 8.5, 1%Luminol, 0.44% Coomaric Acid and 100 mM Tris-HCl pH 8.5, 0.018% H2O2 (1mL of each Solution, selfmade). Exposure time was 60 seconds.

Courtesy of Bettina Mathes, Ludwig Maximilians University Munich, Germany

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