Anti-JA | Jasmonic acid

Product no: AS11 1799

AS11 1799 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Jasmonic acid

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  • Product Info
  • Immunogen:

    BSA-conjugated jasmonic acid (JA)

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 0.5 mg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Immunolocalization (IL)
    Recommended dilution: 1 : 1000 (IL)
  • Reactivity
  • Confirmed reactivity: Populus trichocarpa, Solanum lycopersicum
    Predicted reactivity: Higher Plants
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples

  • immunolocalization using anti-jasmonic acid antibodies

    Material: 
    fully developed leaves of tomato plants (Solanum lycopersicum cv. MicroTom) wounded by squeezing with tweezers.
    The secondary antibody:  goat anti-rabbit IgG coupled to AlexaFluor488 (catalog # A-11008, Invitrogen).

    Immunolocalization was done according to Hause at al. (2013).
    Application examples:

    Reactant: Populus trichocarpa (Black cottonwood)

    Application: Immunohistochemistry-immunofluorescence

    Pudmed ID: 32192046

    Journal: Int J Mol Sci

    Figure Number: 10A,B,C,D,E,F,G,H,I

    Published Date: 2020-03-17

    First Author: Wojciechowska, N., Wilmowicz, E., et al.

    Impact Factor: 5.542

    Open Publication

    Immunodetection of JA (green fluorescence) and red autofluorescence of chloroplasts in leaves during senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E, epidermis. Scale bars = 50 µm.


    Reactant: Populus trichocarpa (Black cottonwood)

    Application: Immunohistochemistry-immunofluorescence

    Pudmed ID: 32192046

    Journal: Int J Mol Sci

    Figure Number: 9A

    Published Date: 2020-03-17

    First Author: Wojciechowska, N., Wilmowicz, E., et al.

    Impact Factor: 5.542

    Open Publication

    Immunolocalization of JA (green fluorescence) in fine, absorptive roots during senescence. (A,B), Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was recorded to visualize the cell and/or organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ph, phloem; X, xylem. Scale bars = 50 µm.


    Reactant: Lupinus luteus (European yellow lupine)

    Application: Immunocytochemistry

    Pudmed ID: 35214860

    Journal: Plants (Basel)

    Figure Number: 4B,C,D

    Published Date: 2022-02-15

    First Author: Domagalski, K.

    Impact Factor:

    Open Publication

    Drought upregulates the level of jasmonic acid (JA) in the abscission zone (AZ) of yellow lupine flowers. AZ fragments were collected for these analyses from 48 day-old plants subjected to drought (25% water holding capacity, WHC) or lupines cultivated in the soil of optimal moisture (70% WHC, control). Details in the Material and Methods section. The level of JA was examined using GC-MS (A). A significant difference is ** p < 0.01. Immunolocalization of JA in the AZ of control (B) or stressed (C,D) plants. Green fluorescence is related to JA localization (indicated by red arrows), whereas blue color corresponds to DAPI-stained nuclei. AZ area is marked by white dotted lines (B,C). (D) image is magnified AZ area presented on C. Abbreviations: AZ—abscission zone; D—distal region of AZ; P—proximal region of AZ. Bars = 40 µM.

  • Additional Information
  • Additional information: This product can be sold containing ProClin if requested
  • Background
  • Background:

    Jasmonic acid (JA) is a member of the jasmonate class of plant hormones and is derived from the fatty acid linolenic acid, biosynthesized from linolenic acid by the octadecanoid pathway. Its main function is regulation of plant response to abiotic and biotic stresses as well as plant growth and development.

  • Product Citations
  • Selected references: Akhiyarova et al. (2024). The Long-Distance Transport of Some Plant Hormones and Possible Involvement of Lipid-Binding and Transfer Proteins in Hormonal Transport. Cells. 2024 Feb 20;13(5):364.doi: 10.3390/cells13050364.
    Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci, 21 (6)
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