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JA | Jasmonic acid

AS11 1799 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Jasmonic acid

JA | Jasmonic acid in the group Antibodies Plant/Algal  / Hormones / Jasmonates at Agrisera AB (Antibodies for research) (AS11 1799)
JA | Jasmonic acid



DATA SHEET IN PDF

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Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

BSA-conjugated jasmonic acid (JA)

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 0,5 mg
Reconstitution For reconstitution add 50 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Immunolocalization (IL)
Recommended dilution 1 : 1000 (IL)

Reactivity

Confirmed reactivity Populus trichocarpa, Solanum lycopersicum
Predicted reactivity Higher Plants
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

immunolocalization using anti-jasmonic acid antibodies

Material: 
fully developed leaves of tomato plants (Solanum lycopersicum cv. MicroTom) wounded by squeezing with tweezers.
The secondary antibody:  goat anti-rabbit IgG coupled to AlexaFluor488 (catalog # A-11008, Invitrogen).

Immunolocalization was done according to Hause at al. (2013).

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 10A,B,C,D,E,F,G,H,I

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunodetection of JA (green fluorescence) and red autofluorescence of chloroplasts in leaves during senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E, epidermis. Scale bars = 50 ĩm.


Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 9A

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunolocalization of JA (green fluorescence) in fine, absorptive roots during senescence. (A,B), Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was recorded to visualize the cell and/or organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ph, phloem; X, xylem. Scale bars = 50 ĩm.

Additional information

Additional information This product can be sold containing ProClin if requested

Related products

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AS16 3688 | Anti-JAR1 | Jasmonic acid-amido synthetase JAR1, rabbit antibodies
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AS12 1854 | Anti-NPR1  Nonexpresser of PR genes 1, rabbit antibodies

Antibodies to plant hormones

Plant and algal protein extraction buffer

Secondary antibodies

Background

Background

Jasmonic acid (JA) is a member of the jasmonate class of plant hormones and is derived from the fatty acid linolenic acid, biosynthesized from linolenic acid by the octadecanoid pathway. Its main function is regulation of plant response to abiotic and biotic stresses as well as plant growth and development.

Product citations

Selected references Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6)

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