JA | Jasmonic acid

AS11 1799 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Jasmonic acid

Product Information

Immunogen

BSA-conjugated jasmonic acid (JA)

Host Rabbit

Clonality Polyclonal

Purity Total IgG. Protein G purified in PBS pH 7.4.

Format Lyophilized

Quantity 0,5 mg

Reconstitution For reconstitution add 50 µl of sterile water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Immunolocalization (IL)

Recommended dilution 1 : 1000 (IL)

Reactivity

Confirmed reactivity Populus trichocarpa, Solanum lycopersicum

Predicted reactivity Higher Plants
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Application example

Material:
fully developed leaves of tomato plants (Solanum lycopersicum cv. MicroTom) wounded by squeezing with tweezers.
The secondary antibody: goat anti-rabbit IgG coupled to AlexaFluor488 (catalog # A-11008, Invitrogen).

Immunolocalization was done according to Hause at al. (2013).

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 10A,B,C,D,E,F,G,H,I

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunodetection of JA (green fluorescence) and red autofluorescence of chloroplasts in leaves during senescence. (A–C) Leaves control (LC); (D–F) The first stage of leaf senescence (LS1); (G–I), The second stage of leaf senescence (LS2). Abbreviations: VB, vascular bundle; PM, palisade mesophyll; SM, spongy mesophyll; E, epidermis. Scale bars = 50 µm.

Reactant: Populus trichocarpa (Black cottonwood)

Application: Immunohistochemistry-immunofluorescence

Pudmed ID: 32192046

Journal: Int J Mol Sci

Figure Number: 9A

Published Date: 2020-03-17

First Author: Wojciechowska, N., Wilmowicz, E., et al.

Impact Factor: 5.542

Open Publication

Immunolocalization of JA (green fluorescence) in fine, absorptive roots during senescence. (A,B), Root control (RC); (C,D) The first stage of root senescence (RS1); (E,F) The second stage of root senescence (RS2). Autofluorescence (red) of the cell wall was recorded to visualize the cell and/or organ shape. Abbreviations: R, rhizodermis; C, parenchyma cortex cells; Ph, phloem; X, xylem. Scale bars = 50 µm.

Additional information

Additional information This product can be sold containing ProClin if requested

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Secondary antibodies

Background

Jasmonic acid (JA) is a member of the jasmonate class of plant hormones and is derived from the fatty acid linolenic acid, biosynthesized from linolenic acid by the octadecanoid pathway. Its main function is regulation of plant response to abiotic and biotic stresses as well as plant growth and development.

Product citations

Selected references Wojciechowska et al. (2020). Abscisic Acid and Jasmonate Metabolisms Are Jointly Regulated During Senescence in Roots and Leaves of Populus trichocarpa. Int J Mol Sci , 21 (6)