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JAZ1 | Jasmonate ZIM-domain protein 1

AS13 2648 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

JAZ1 | Jasmonate ZIM-domain protein 1  in the group Antibodies for Plant/Algal  / Hormones / Jasmonates at Agrisera AB (Antibodies for research) (AS13 2648)

DATA SHEET IN PDF

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Product Information

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAZ1 protein sequence, UniProt: Q9LMA8, TAIR: AT1G19180

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.5
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 1 : 1000-1 : 3000 (WB)
Expected | apparent MW

27.6 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in

Oryza sativa, Solanum tuberosum

Application examples

Application examples

application example

western blot using anti-JAZ1 antibodies

JAZ1HA was transiently expressed in Arabidopsis thaliana protoplast for 6 hours. 200 µl of protoplast cells were harvested and mixed with 100 µl 2X SDS buffer. Proteins were separated on 4-12% NuPAGE gel and blotted for 1 hr to PVDF using the Invitrogen XCell II blot module. Blot was blocked with 5% non-fat milk in TBST buffer for 1 hr at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 over night at 4°C with agitation in TBST with 5% non-fat milk. The blot was rinsed briefly twice, then washed 3 times with TBST for a total of 45 min at RT. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1: 15 000 for 1 hr at RT in TBST with 5% non-fat milk. The blot was washed as above and developed for 5 min using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and exposed for 2 min.

Courtesy of Dr. Xuecheng Zhang, Department of Molecular Biology Massachusetts General Hospital, USA

Additional information

Related products

Related products

AS12 2637 | anti-COI1 | Coronate insensitive 1, rabbit antibodies

Plant protein extraction buffer

Secondary antibodies

Background

Background

JAZ1 (Jasmonate-ZIM domain containing protein 1)  acts as a repressor of jasmonate responses which are negatively regultated by the proteasome. Interacts with COI1 and inositol pentakisphosphate.

Product citations

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