JAZ1 | Jasmonate ZIM-domain protein 1

Product no: AS13 2648

AS13 2648 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen:

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana JAZ1 protein sequence, UniProt: Q9LMA8, TAIR: AT1G19180

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 1000-1 : 3000 (WB)
    Expected | apparent MW: 27,6 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in:

    Gossypium hirsutum, Oryza sativa, Solanum tuberosum

  • Application Examples
  • application example

    western blot using anti-JAZ1 antibodies

    JAZ1HA was transiently expressed in Arabidopsis thaliana protoplast for 6 hours. 200 µl of protoplast cells were harvested and mixed with 100 µl 2X SDS buffer. Proteins were separated on 4-12% NuPAGE gel and blotted for 1 hr to PVDF using the Invitrogen XCell II blot module. Blot was blocked with 5% non-fat milk in TBST buffer for 1 hr at room temperature with agitation. Blot was incubated in the primary antibody at a dilution of 1: 3000 over night at 4°C with agitation in TBST with 5% non-fat milk. The blot was rinsed briefly twice, then washed 3 times with TBST for a total of 45 min at RT. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1: 15 000 for 1 hr at RT in TBST with 5% non-fat milk. The blot was washed as above and developed for 5 min using the SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and exposed for 2 min.

    Courtesy of Dr. Xuecheng Zhang, Department of Molecular Biology Massachusetts General Hospital, USA
  • Background
  • Background:

    JAZ1 (Jasmonate-ZIM domain containing protein 1)  acts as a repressor of jasmonate responses which are negatively regultated by the proteasome. Interacts with COI1 and inositol pentakisphosphate.

  • Product Citations
  • Selected references: 38221900
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS12 2637 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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AS16 ECL-S | extreme low femtogram detection

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