LacI | Lactose operon repressor (clone 15C11)
AS16 3144 | Clonality: Monoclonal | Host: Mouse | Reactivity: LacI from E.coli and A. thaliana

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Product Information
Immunogen
Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli
Host
Mouse
Clonality
Monoclonal
Subclass/isotype
IgG1
Purity
IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.
Format
Liquid
Quantity
100 ĩg
Storage
Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
5 µg in 10 ml (WB)
Expected | apparent MW
45 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Escherichia coli
Predicted reactivity
Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
Species of your interest not listed? Contact us
Species of your interest not listed? Contact us
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Additional information
Additional information
This antibody recognizes recombinant and native Lacl
Background
Background
LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
Product citations
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