LacI | Lactose operon repressor (clone 15C11)

Name: LacI | Lactose operon repressor (clone 15C11)
Brand: IKP
SKU: AS16 3144
Price: 421 EUR
Availability: InStock

AS16 3144 | Clonality: Monoclonal | Host: Mouse | Reactivity: LacI from E.coli and A. thaliana

LacI | Lactose operon repressor (clone 15C11) in the group Antibodies Other Species / Bacteria at Agrisera AB (Antibodies for research) (AS16 3144)

Product Information

Immunogen Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli

Host Mouse

Clonality Monoclonal

Subclass/isotype IgG1

Purity IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.

Format Liquid

Quantity 100 µg

Storage Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications Western blot (WB)

Recommended dilution 5 µg in 10 ml (WB)

Expected | apparent MW

45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Escherichia coli

Predicted reactivity Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
Species of your interest not listed? Contact us

Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Additional information

Additional information This antibody recognizes recombinant and native Lacl

Background

Background LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.

Product citations

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immunogen:	Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli
Sub class:	IgG1
Host:	Mouse
Clonality:	Monoclonal
Purity:	IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.
Format:	Liquid
Quantity:	100 µg
storage:	Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	Western blot (WB)
recommended dilution:	5 µg in 10 ml (WB)
calculated \| apparent molecular mass [kDa]:	45 kDa
Confirmed reactivity:	Arabidopsis thaliana, Escherichia coli
predicted reactivity:	Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp. Species of your interest not listed? Contact us
not reactive in:	No confirmed exceptions from predicted reactivity are currently known
additional information:	This antibody recognizes recombinant and native Lacl
background:	LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.