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LacI | Lactose operon repressor (clone 15C11)

AS16 3144  | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: LacI from E.coli and A. thaliana

LacI | Lactose operon repressor (clone 15C11) in the group Antibodies, Bacterial/Fungal at Agrisera AB (Antibodies for research) (AS16 3144)

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Product Information

Immunogen Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E. coli
Host Mouse
Clonality Monoclonal
Subclass/isotype IgG1
Format Liquid in 0.1M sodium phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) sodium azide
Quantity 100 µg
Storage Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Recommended dilution 5 µg in 10 ml (WB)
Expected | apparent MW

45 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Escherichia coli
Predicted reactivity Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known.

Application examples

Additional information

Additional information

This antibody recognizes recombinant and native Lacl.

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Background

Background LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.

Product citations

Selected references Kim T., et al. (2005) Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 3(6):571-82.

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