IKP

LacI | Lactose operon repressor (clone 15C11)

Product no: AS16 3144

AS16 3144  | Clonality: Monoclonal  |  Host: Mouse  |  Reactivity: LacI from E.coli and A. thaliana

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Delivery:  7-10 business days
  • Product Info
  • Immunogen: Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli
    Sub class: IgG1
    Host: Mouse
    Clonality: Monoclonal
    Purity: IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.
    Format: Liquid
    Quantity: 100 g
    Storage: Store at -20C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 5 µg in 10 ml (WB)
    Expected | apparent MW:

    45 kDa

  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana, Escherichia coli
    Predicted reactivity: Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp.
    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information: This antibody recognizes recombinant and native Lacl
  • Background
  • Background: LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
  • Product Citations
  • Selected references: Kim T., et al. (2005) Engineering a root-specific, repressor-operator gene complex. Plant Biotechnol J. 3(6):571-82.
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